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Öğe Amiodaron’un Neden Olduğu Sıçan Doku Toksisitesinde Astaksantin’in Antioksidan Etkinliği(2023) Kara, Ozlem; Kilitci, AsumanAmaç: Astaksantin'in amiodaronun neden olduğu böbrek dokusu hasarı üzerindeki etkisini değerlendirmeyi amaçladık. Yöntemler: 30 adet Wistar albino rat kullanılarak 3 grup oluşturuldu. Grup 1'de (kontrol grubu) (n=10) herhangi bir ilaç verilmedi ve herhangi bir işlem yapılmadı. Grup 2'ye (amiodaron grubu) (n=10) 100 mg/kg amiodaron 7 gün verildi. Grup 3'e (amiodaron+astaksantin grubu) (n=10) 100 mg/kg amiodaron ve 25 mg/kg astaksantin 7 gün verildi. Tüm gruplarda sağ böbrekler cerrahi olarak çıkarıldı. Kan malondialdehit (MDA) seviyeleri ve katalaz (CAT) ve süperoksit dismutaz (SOD) aktiviteleri ölçüldü. Ayrıca böbrek dokusundan hazırlanan lamlar mikroskopi ile incelenerek damar tıkanıklığı, kanama, tübül dejenerasyonu ve glomerüler hasar gibi toksisite belirteçleri değerlendirildi. Bulgular: Grup 2'de MDA düzeyleri grup 3'e göre anlamlı olarak daha yüksek, SOD ve CAT aktiviteleri daha düşüktü (p<0.05). Doku hasarı grup 2'de grup 3'e göre anlamlı olarak yüksekti (p<0,05). Sonuç: Kısa vadeli bulgularımıza göre astaksantin, amiodaronun böbrek dokusu üzerindeki toksisitesini tersine çevirmiştir.Öğe Comprehensive bronchoalveolar lavage characterization in COVID-19 associated acute respiratory distress syndrome patients: a prospective cohort study(Bmc, 2023) Calabrese, Fiorella; Lunardi, Francesca; Baldasso, Elisa; Pezzuto, Federica; Kilitci, Asuman; Olteanu, Gheorghe-Emilian; Del Vecchio, ClaudiaCOVID-19-related acute respiratory distress syndrome (CARDS) is associated with high mortality rates. We still have limited knowledge of the complex alterations developing in the lung microenvironment. The goal of the present study was to comprehensively analyze the cellular components, inflammatory signature, and respiratory pathogens in bronchoalveolar lavage (BAL) of CARDS patients (16) in comparison to those of other invasively mechanically ventilated patients (24). In CARDS patients, BAL analysis revealed: SARS-CoV-2 infection frequently associated with other respiratory pathogens, significantly higher neutrophil granulocyte percentage, remarkably low interferon-gamma expression, and high levels of interleukins (IL)-1 beta and IL-9. The most important predictive variables for worse outcomes were age, IL-18 expression, and BAL neutrophilia. To the best of our knowledge, this is the first study that was able to identify, through a comprehensive analysis of BAL, several aspects relevant to the complex pathophysiology of CARDS.Öğe Demographic and Histopathological Features of Oral Mucosa Lesions in Kirşehir Region: A Retrospective Cross-Sectional Study(OrtadogŸu Reklam Tanitim Yayincilik Turizm Egitim Insaat Sanayi ve Ticaret A.S., 2022) Acar, Emine Müge; Kilitci, AsumanObjective: The aim of this study was to evaluate the histopathological findings of the excised lesions from oral mucosa and determine the type and frequency of oral mucosa diseases in Kirşehir region. Material and Methods: The histopathology results of 237 patients with oral mucosa lesions who administered to Kirşehir Training and Research Hospital Dermatology Outpatient Clinic and underwent incisional or excisional biopsy between December 2014 and July 2018 were retrospectively evaluated. The demographic characteristics of the patients, disease duration, localisation and frequency of the lesions, the frequency of benign and malignant lesions were recorded. Results: Ninety three (39.2%) male and 144 (60.8%) female patients were recruited in our study. The mean age of the patients was 44.33±1.21 years. A total of 237 oral mucosa lesions were detected. The most common benign lesions were intradermal nevi (n=56, 23.6%) followed by inflammatory granulation tissue (n=25, 10.5%), fibromas (n=26, 11%), mucocele (n=21, 8.9%), pyogenic granuloma (n=16, 6.7%), irritation fibromas (n=14, 5.9%), squamous papilloma (n=8, 3.4%), verruca vulgaris (n=6, 2.5%), lichen planus (n=6, 2.5%), hemangiomas (n=5, 2.1%). The most common malignant lesions were squamous cell carcinoma (n=9, 3.8%) and basal cell carcinoma (n=7, 2.9%), followed by lymphoma (n=1, 0.4%) and basosquamous cell carcinoma (n=1, 0.4%). Conclusion: In our study, the vast majority (92.8%) of the lesions detected in oral mucosa were benign lesions while malignant lesions constituted a small proportion. Gaining knowledge about the distribution of oral mucosal diseases may contribute to prevention and treatment of these diseases. © 2022 OrtadogŸu Reklam Tanitim Yayincilik Turizm Egitim Insaat Sanayi ve Ticaret A.S.. All rights reserved.Öğe The effect of astaxanthin on amiodarone induced cardiac tissue damage in rat(Cukurova Univ, Fac Medicine, 2023) Kara, Ozlem; Kilitci, AsumanPurpose: The aim of this study was to evaluate the effect of astaxanthin on amiodarone induced cardiac tissue damage.Materials and Methods: 3 groups were formed using 30 Wistar albino rats. In group 1 (control group) (n=10), neither any drugs were given nor anything was performed. In group 2 (amiodarone group) (n=10), 100 mg/kg amiodarone was given for 7 days. In group 3 (amiodarone+astaxanthin group) (n=10), 100 mg/kg amiodarone and 25 mg/kg astaxanthin were given for 7 days. Hearts were surgically extirpated in all groups. Blood malondialdehyde (MDA) levels and activities of catalase (CAT) and superoxide dismutase (SOD) were measured. Also, toxicity markers such as edema, hemorrhage, fibrosis, inflammatory cell infiltration were assessed by examining the slides prepared from cardiac tissue with microscopy.Results: The MDA levels were significantly higher and the activities of SOD, and CAT were lower in group 2 than group 3. Tissue damage was significantly higher in group 2 than group 3.Conclusion: According to our short term findings, astaxanthin reversed the toxicity of amiodarone on cardiac tissue. In the light of these promising results, we suggest that astaxanthin usage should be thought to protect the cardiac damage due to amiodarone.Öğe The effect of radiofrequency electromagnetic radiation on rat liver tissue and serum paraoxonase (PON1)(2023) Yavaş, Mehmet Cihan; Kilitci, AsumanAim: The development of technology increases the rate of everyone having a smartphone. Therefore, the possible biological effects of these devices are of concern. In this study, we aimed to investigate the effect of 2100 MHz radiofrequency electromagnetic radiation on rat liver tissue and serum paraoxonase 1 level. Materials and Methods: Within the scope of the study, a 2100 MHz radiofrequency electromagnetic radiation model was created. In our study, we used Sprague Dawley male rats. Two groups were made as sham-control and exposure group (5 h a day for 2 weeks). Liver tissue and serum paraoxonase were studied. Results: The paraoxonase 1 value of the exposure group was higher than the sham control group, and did not have statistically important difference in the comparison of exposure and sham control groups (p>0.05). Did not have important difference in histopathological parameters of rat liver tissue (p>0.05). Conclusion: Although it seems that radiofrequency radiation does not cause liver damage, more detailed studies with short- and long-term exposure are needed.Öğe Healing Effects of Single-Dose Triptolide in Rats with Severe Acute Pancreatitis(2020) Dulkadiroğlu, Erkan; Yıldız, Ahmet; Karaca, Gökhan; Şahin, Yaşar; Özden, Hüseyin; Kilitci, Asuman; Umudum, HaldunAim: Severe acute pancreatitis (SAP) carries high morbidity and mortality risk. If theproinflammatory response phase of SAP cannot be controlled, it may result in multiorganfailure (MOF). Nuclear factor-kappa B (NF-?B) activation plays an important role in thedevelopment of MOF. In this study, it was aimed to investigate the healing effects of triptolide,an anti-inflammatory and immunosuppressive agent in rats with SAP.Material and Methods: A total of 20 Wistar-Albino rats were divided into two groups as theSAP and triptolide treatment (TT) groups. SAP was induced by intraperitoneal injection ofcerulean (50 mg/kg) in both groups. TT group was administered a single dose (0.2 mg/kg)triptolide 24 hour after the induction of SAP. Serum ALT, AST, GGT, Lipase, Glucose, ALPand amylase levels and pancreatic tissue samples were examined.Results: Serum glucose and amylase levels were found to be significantly lower in the TTgroup (p=0.011 and p=0.035, respectively). There was no significant difference between thegroups in terms of other biochemical parameters. Pancreatic edema, acinar cell degeneration,fat necrosis, intrapancreatic&perivascular inflammation, inflammation in the peripancreatic fattissue were common histopathological findings in both groups. There was no significantdifference between the groups in terms of histopathologic changes.Conclusion: Cerulein-induced pancreatitis is a successful method for experimental SAP. Thehealing effects of single-dose triptolide treatment are not evident in the early phase of SAP.The therapeutic effects of triptolide on inflammatory and oxidative stress were not significantlyapproved by histopathological and biochemical parameters by the pancreatic tissue.Öğe Intraoperative Dermatoscopic Features of Subungual Exostosis(Mashhad Univ Med Sciences, 2020) Elmas, Omer Faruk; Kilitci, Asuman; Uyar, Belkiz; Gamsizkan, Mehmet[Abstract Not Available]Öğe Melanocytes as the source of the increased melanisation in pigmented epithelial tumours: a holistic approach(Tubitak Scientific & Technical Research Council Turkey, 2022) Kilitci, Asuman; Elmas, Ömer Faruk; Akdeniz, Necmettin; Gamsızkan, MehmetBackground/aim: We aimed to elucidate the causes of the increased melanisation in basal cell carcinoma (BCC) and seborrheic keratosis (SK), and the role of melanocytes in this process. Materials and methods: This study was a retrospective-cohort study conducted in the pathology department of a university hospital between January 2019 and October 2020. Forty-nine SK and 30 pigmented BCC were included in our study. SRY-box transcription factor 10 (SOX10), CD68, and Masson???Fontana staining was used for analysis in all samples. A representative section of each specimen was photographed under ??400 magnification to facilitate the assessments of the morphology of the melanocytes and their following morphometric parameters: density, nuclear diameter, and distribution. The density of pigmented keratinocytes in the lesional epidermis was scored. The nuclear diameters of melanocytes located in the nonlesional epidermis, the density of the melanophages, and the presence or absence of ulceration and solar elastosis were also recorded. Results: The morphometric findings confirmed a statistically significant increase in melanocyte density in the BCC group compared with that in the SK group (p < 0.001). Moreover, the nuclear minor diameters in the melanocytes of the BCC sections were significantly higher than those in the SK specimens (p < 0.001). The epidermal melanocytes were distributed diffusely in almost all BCC specimens (96.7%), whereas they were mainly limited to the basal layer in the majority of the SK sections (59.2%). The number of epidermal melanised keratinocytes with a score of 3 was significantly higher in the SK group (n = 31; 63.2%) than in the BCC group (n = 6; 20%) (p = 0.001), and they were the main cells representing the pigmented appearance of the tumours. No significant difference was found between both tumour groups in terms of their melanophage density scores (p = 0.206). Conclusion: This study is the first step towards an objective quantification of the melanocytes in pigmented epithelial tumours and may provide a morphological background for future studies on these skin lesions.Öğe Possible Effect of 2100 Mhz Cell Phone Radiation on Heart and Spleen Tissues of Rats(Gazi Univ, Fac Med, 2022) Yavas, Mehmet Cihan; Kilitci, Asuman; Yegin, Korkut; Oruç, Sinem; Delen, Kevser; Sirav, BahriyeIntroduction: The widespread access of the society to mobile phones and their intensive use cause health concerns. This study is aimed to investigate the possible histopathological effects of exposure to Radio-frequency Radiation (RFR) originating from mobile phones rat heart and spleen tissue. Methods: Totally 14 Sprague Dawley male rats were used and divided into 2 group (n= 7 for each); sham-control group, exposure group. RFR exposure group was exposed to 2100 MHz GSM-like RFR for 5 hours/day, for 14 days. Heart and spleen tissues of all rats were sampled for histopathological examination and hematoxylin-eosin staining was performed. For p53 examination, immunohistochemical staining was also used in both tissues. Results: Histopathological and immunohistochemical evaluation revealed no significant changes in heart tissue, but trabecular irregularity and enlargement of sinusoids were observed in the spleen in histopathological evaluation. Conclusion: It was observed that short-term RFR exposure did not cause significant morphological changes in heart and spleen tissue. This situation may be seen due to the protective effect of the body against RFR.Öğe Prevalence of High-Risk Human Papillomavirus Types and Their Association with Cervical Squamous Cell Carcinoma, and High- and Low-Grade Squamous Intraepithelial Lesions in Turkish Women(Iranian Scientific Society Medical Entomology, 2023) Erkinueresin, Taskin; Kartal, Esra; Ustunyurt, Emin; Demirci, Hakan; Kilitci, Asuman; Onal, BinnurBackground: This study aimed to investigate human papillomavirus (HPV) type prevalence in our region and the relationship between uterine cervical HPV types and squamous cell carcinoma (SCC)/intraepithelial lesions.Methods: HPV test results were obtained from patient file archives of the Gynecology and Obstetrics Clinic. Pathology report results were obtained from the digital records of the Pathology Laboratory and the patient file archives of the Gynecology and Obstetrics Clinic in 2018.Results: The most frequently detected was HPV16 (29.2%), followed by HPV51 (13.1%), HPV56 (11%), HPV31 (9.2%), HPV52 (8.4%), and HPV68 (8.2%). HPV16 was positive in 50% of patients diagnosed with SCC, 54.9% of patients with HSIL, 27% of patients with LSIL, and 25% of cases diagnosed as benign (P<0.001). HPV18 was positive in 25% of patients diagnosed with SCC, 11% of patients with HSIL, 4.7% of patients with LSIL, and 5.2% of cases diagnosed as benign (P=0.019).Conclusion: The most frequent hrHPV genotype was HPV16, followed by HPV51, HPV 56, and HPV 31. There is an increase in the frequency of HPV51 and HPV56. HPV51, not included in HPV vaccines and is the second most frequent, should be included in these vaccines. In addition, although the frequency of HPV18 has decreased, HSIL and SCC generation is still high together with HPV16.Öğe Protective effect of resveratrol on cisplatin induced damage in rat kidney(Cukurova Univ, Fac Medicine, 2022) Kara, Özlem; Kilitci, Asuman; Dağlıoğlu, GülçinPurpose: The aim of this study was to evaluate the protective effect of resveratrol on cisplatin induced damage in rat kidney. Materials and Methods: 30 female Wistar-Albino rats were allocated to form three groups: In group 1 (control group), 1 mL of 0.9% NaCl (saline) was administered intraperitoneally for 3 days. In group 2 (cisplatin group), 7.5 mg / kg intraperitoneal cisplatin was given for 3 days. In group 3 (cisplatin + resveratrol group) 7.5 mg / kg cisplatin and 10 mg / kg resveratrol were given via intraperitoneal route. Right kidneys were surgically extirpated in all groups. Malondialdehyde (MDA) levels and activities of catalase (CAT) and superoxide dismutase (SOD) were measured in both blood and tissues. Also, toxicity markers such as vascular congestion, hemorrhage, tubule degeneration and glomerular damage were assessed by examining the slides prepared from kidney tissue with microscopy. Results: Tissue damage was significantly higher in group 2 than other groups. The MDA levels were significantly higher and the activities of SOD, and CAT were lower in group 2 than other groups. Conclusion: According to our short term findings, resveratrol might be an effective molecule to prevent the harmful effect of cisplatin in rat kidney.Öğe The Protective Effect of Resveratrol on Cisplatin Induced Damage in Rat Liver(Galenos Publ House, 2022) Kara, Özlem; Kilitci, AsumanAim: One of the underlying causes of cisplatin-induced hepatotoxicity is oxidative stress. We assessed the effect of an antioxidant, resveratrol, on cisplatin-induced damage in the rat liver. Methods: The project-starting date was designed as 01.10.2020 and the project-ending date was planned as 01.04.2021. Three groups were created with 30 female Wistar-Albino rats: In group 1 (control group), 1 mL of 0.9% NaCl (saline) was administered intraperitoneally for 3 days. In group 2 (cisplatin group), 7.5 mg/kg intraperitoneal cisplatin was given for 3 days. In group 3 (cisplatin + resveratrol group), 7.5 mg/kg cisplatin and 10 mg/kg resveratrol were given via the intraperitoneal route. The livers were surgically extirpated in all the groups. In both blood and tissues, malondialdehyde (MDA) levels and activities of catalase (CAT) and superoxide dismutase (SOD) were measured. Also, toxicity markers such as hepatocyte damage (cellular changes), inflammation, hemorrhage, congestion, fibrosis, disorganization of the hepatic cords, and necrosis were assessed by examining the preparations prepared from hepatic tissue with light microscopy and immunohistochemistry. Results: Histopathological tissue damage was significantly higher in group 2 than in other groups (p 0.03). MDA levels were significantly higher and the activities of SOD and CAT were lower in group 2 than in the other groups (p=0.04 and p=0.01, respectively). Conclusion: According to our short-term findings, resveratrol might be an effective molecule for preventing the harmful effects of cisplatin in the rat liver.Öğe Refining diagnosis is the prerequisite for the correct treatment: The cytodiagnostic utility of SOX10 in the diagnosis of metastatic melanoma(Wiley, 2021) Kilitci, Asuman; Elmas, Omer Faruk; Demirbas, Abdullah; Gamsizkan, Mehmet; Atasoy, Mustafa; Tursen, Umit; Lotti, Torello[Abstract Not Available]
