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  • Küçük Resim Yok
    Öğe
    İki Farklı COVID-19 Aşısı Sonrası Oluşan IFN-γ ve IgG Antikor Düzeylerinin Karşılaştırılması
    (2025) Dönmez, Betül; Öksüz, Şükrü; Calıskan, Emel; Öztürk, C. Elif; Ege, Nagihan; Acar, Eda; Keskin, Banu Hümeyra
    Amaç: Çalışmamızın hedefi, CoronaVac ile BioNTech aşı uygulamalarından sonra meydana gelen, hücresel ve hümoral bağışıklığın belirteci olan interferon-gama ile immünoglobulin G antikor seviyelerinin kıyaslanmasıdır. Yöntem: Çalışmamızda ikişer doz olarak CoronaVac aşısı uygulanmış 18 gönüllü ve BioNTech aşısı uygulanmış 22 gönüllünün serumlarından Anti-SARS-CoV-2 IGRA testi ile IFN-γ cevabı ve SARS-CoV-2 IgG II Quant Reagent testi ile IgG antikor cevabı araştırılmıştır. Bulgular: Araştırmamıza dahil edilen aşı grupları arasında IFN-γ yanıtı açısından anlamlı seviyede fark tespit edilirken (p=0.007), IgG antikor yanıtı açısından ise anlamlı bir fark tespit edilmemiştir (p>0.05). Her iki aşı grubundaki immün yanıtlar kantitatif olarak değerlendirildiğinde ise, IFN-γ ve IgG antikor yanıtı BioNTech aşı grubunda, CoronaVac aşı grubunda tespit edilen sonuçlardan anlamlı seviyede daha yüksek saptanmıştır (sırasıyla; p<0.001, p<0.001). Sonuç: İnterferon gama salınım testinin(IGRA) temel avantajı, özel ekipmana gereksinim olmadan ve çok kısa süre içerisinde gerçekleştirilme imkanı olmasıdır. COVID-19’da aşı cevabı için antikor tetkikleri sıkça kullanılmasına karşın, T hücre yanıtını tespit eden Anti-SARS-CoV-2 IGRA tetkiki de güvenilir bir metod olarak kullanılabilmektedir.
  • Küçük Resim Yok
    Öğe
    Investigation of Isoniazid, Rifampicin and Second Generation Antibiotic Resistance Genes in Rifampicin-Resistant Mycobacterium tuberculosis Complex Strains Isolated at Düzce University Between 2004-2021
    (Duzce Univ, Fac Medicine, 2025) Akbas, Emel; Ege, Nagihan; Atik, Dursun; Caliskan, Emel; Oksuz, Sukru; Sahin, Idris; Ozturk, Elif Cihadiye
    Objective: To determine the gene patterns causing antibiotic resistance in M. Methods: Nineteen rifampicin-resistant MTBC strains isolated between 2004 and 2021 were included. The species of these strains with MTBC genotype and the gene pattern causing rifampicin resistance with MTBDR plus genotype were analysed. Results: Nineteen of the isolates were identified as M. tuberculosis/canetti by the MTBC genotype method. Seven of these isolates were genotypically resistant to rifampicin. One of the resistant isolates had deletion in WT8 and WT6 bands, one had deletion in WT8 band, one had deletion in WT7 band and rpoBMUT2A mutation, and four had deletion in WT8 band and rpoBMUT3 mutation. Seven of the resistant isolates were genotypically INH resistant. Five of them had katGMUT1 mutation with deletion in katGWT band and two of them had only INH AMUT3B mutation. Of the 10 multidrug-resistant MTBC isolates, nine were genotypically resistant to none of the second-generation drugs using the GenoType MTBDR sl ver 2.0 method. However, one isolate could not be evaluated with this assay. Conclusions: The presence of MDR-TB and RR-TB is an important challenge especially in TB control, which increases the need for molecular methods. Although it still has not replaced culture, there is a need for the use and development of new molecular methods that will benefit us in TB treatment and control.
  • Küçük Resim Yok
    Öğe
    Investigation of the relationship between fluconazole susceptibility, proteinase activity and ERG11-SAP2 Expression in Candida albicans strains isolated from clinical samples
    (Mazandaran University of Medical Sciences, 2024) Ege, Nagihan; Oksuz, S.; Akbaş, Emel; Çalişkan, Emel; Sungur, Mehmet Ali
    Background and Purpose: Candida albicans is currently recognised as an opportunistic pathogen that can cause many invasive infections. Resistance mechanisms and fungal virulence factors play an important role in the effectiveness of treatment. The aim of this study was to investigate the relationship between fluconazole resistance, proteinase activity and ERG11 (sterol 14-demethylase)- SAP2 (secreted aspartic protease 2) gene expression levels in C.albicans strains. Materials and Methods: Candida albicans strains isolated from patient samples sent to Medical Microbiology laboratory of Düzce University from various clinics were included in the study. Fluconazole susceptibilities of the isolates were determined by broth microdilution method. The increase in fluconazole MIC values at 48 hours and proteinase activities of the isolates were analysed. ERG11 and SAP2 gene expression levels were measured by real time qPCR. Results: Fluconazole resistance rate was found to be 3.14% in 127 C. albicans strains. A moderate positive correlation was found between ERG11 and SAP2 values (p=0.029, r:0.655, p<0.001). There was no correlation between SAP2/ERG11 expression levels and fluconazole resistance. Proteinase positivity was detected in 81.1%, of 127 strains and no statistically significant correlation was found between proteinase activities and SAP2/ERG11 expression levels. While there was a statistically significant relationship between ERG11 expression levels and 48th hour MIC elevation, there was no statistically significant relationship between SAP2 levels and 48th hour MIC elevation. Conclusion: In addition to the moderate positive correlation between ERG11 and SAP2 values, a significant correlation was found between ERG11 expression and fluconazole tolerance. © 2025 Elsevier B.V., All rights reserved.