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Öğe Genetic Diversity Among Historical Olive (Olea europaea L.) Genotypes from Southern Anatolia Based on SSR Markers(Springer/Plenum Publishers, 2016) Sakar, Ebru; Ünver, Hülya; Ercişli, SezaiOlive (Olea europaea) is an ancient and important crop in both olive oil production and table use. It is important to identify the genetic diversity of olive genetic resources for cultivar development and evaluation of olive germplasm. In the study, 14 microsatellite markers (UDO4, UDO8, UDO9, UDO11, UDO12, UDO22, UDO24, UDO26, UDO28, DCA9, DCA11, DCA13, DCA15, and DCA18) were used to assess the genetic variation on 76 olive (Olea europaea L.) genotypes from Mardin province together with 6 well-known Turkish and 4 well-known foreign reference cultivars. All microsatellite markers showed polymorphism and the number of alleles varied between 9 and 22, with an average of 14.57. The most informative loci were DCA 11 (22 alleles) and DCA 9 (21 alleles). Dendrogram based on genetic distances was constructed for the 86 olive genotypes/cultivars, which revealed the existence of different clusters. The high genetic similarity was evident between Bakirkire2 and Zinnar5 (0.74) genotypes, while the most genetically divergent genotypes were Gurmese5 and Yedikardes, ler2 (0.19). It was concluded that there was abundant SSR polymorphism in olive germplasm in southern Anatolia in Turkey and could be important for future breeding activities.Öğe Genetic Diversity and Relationships among Local Olive (Olea europeaea L.) Genotypes from Gaziantep Province and Notable Cultivars in Turkey, Based on SSR Markers(Univ Agr Sci & Veterinary Med Cluj-Napoca, 2016) Sakar, Ebru; Ünver, Hülya; Ulaş, Mehmet; Lazovic, Biljana; Ercişli, SezaiOlive and olive oil have a prominent place in the cultures of the countries within the Mediterranean basin including Turkey. The genetic relationships among 30 olive (Olea europaea L.) genotypes sampled from Gaziantep province in Turkey were examined using 10 simple sequence repeat (SSR) markers (DCA9, DCA11, DCA15, DCA18UD04, UDO9, UD011, UDO12, UD022, UD024). Also, three well known Turkish and one foreign olive cultivar were also included within the SSR analysis. The number of alleles per locus of the SSR markers ranged from 5 (DCA15, UDO9) to 14 (DCA9) (average 7.9), for a total of 79 alleles. Similarity coefficients were calculated on the basis of 79 amplified bands. A dendrogram was created according to the 10 SSR markers by the unweighted pair-group method. The banding patterns obtained from the SSR primers allowed all of the genotypes/cultivars to be distinguished. According to the dendrogram, the 33 olive genotypes and cultivars were clustered into five main clusters. The most closely related genotypes were 'Oguzeli 3' and Tavuzeli with 0.80 similarity ratio. The most genetically divergent cultivars were 'Yavuzeli 6' and `Kilis Yaglik' (0.30), Tavuzeli 6' and 'Sauranis (0.20), Nizip 7' and Tavuzeli 4' (0.15), Islahiye 5' and Nizip Yaglik' (0.10). In conclusion, SSR analysis can be an efficient method for olive genotypes and cultivar identification and can offer valuable informative data to identify olive genotypes and cultivars grown in Turkey.Öğe MOLECULAR CHARACTERIZATION OF LOCAL OLIVE GENOTYPES FROM SOUTHERN ANATOLIA FORESTS(Publ House Bulgarian Acad Sci, 2018) Sakar, Ebru; Özkaya, Mücahit Taha; Ergül, Ali; Ünver, Hülya; Ulaş, Mehmet; Toteva, Veneta Kapchina; Ercişli, SezaiTurkey is one of the major olive (Olea europeaea L.) producers in the world and the country has a wide variety of olive germplasm including local genotypes, local cultivars, and standard cultivars as well. However, there is little information on genetic structure of local olive genotypes that are extensively grown especially in southern Anatolia region in Turkey. We examined the genetic relatedness of 58 local Turkish olive genotypes sampled from forests of Sirnak province located in southern Anatolia by using 10 SSR (simple sequence repeat) markers that were previously developed for olive and widely used in different laboratories to assess genetic diversity and relatedness in olive. In the study 4 introduced foreign cultivars along with 6 well-known standard Turkish cultivars are also included in SSR analysis to make comparison with local genotypes. Allele diversity ranged from 8 (UDO9) to 24 (DCA9), (DCA18) an average 17.6 per SSR loci presenting high polymorphism and were found to be higher than in previous studies that used the same loci for olive. No relationship was found between the genetic relatedness and the geographical distributions of these genotypes and cultivars. The data reported here may be useful to prevent confusions of Turkish olive germplasm and could be important in future breeding activities.Öğe Molecular characterization of ancient olive genotypes from Hatay Province in Turkey(Tubitak Scientific & Technical Research Council Turkey, 2016) Sakar, Ebru; Ünver, HülyaTurkey's average share of world olive production is between 7% and 10% and the country is the fourth biggest table olive and olive oil producer in the world. More than fifty olive cultivars have been commercialized in Turkey and there are numerous olive genotypes in different olive-growing regions in Turkey that differ from each other in terms of leaf, flower, and fruit characteristics. The aim of the present study was to identify the 40 most widely grown olive genotypes in Hatay Province in Turkey using microsatellite or simple sequence repeat (SSR) markers. Ten SSR loci were selected and used to identify olive genotypes/cultivars. The number of alleles per locus was found to be between 4 (UDO4 and DCA13) and 16 (DCA9), indicating high polymorphism among olive germplasms. We did not determine identical cultivars in SSR analysis. Samandag2 and Dortyol7 (0.85), Samandag7 and Saurani (0.75), Payas kalesi and Sari ulak (0.75), and Yayladag4 and Samandag3 (0.70) genotype pairs showed higher similarity while Yayladag1 and Samandag8 (0.15), Reyhanli1 and Yayladag6 (0.15), and Samandag8 and Hassa5 (0.15) were found to be the most genetically divergent genotypes.Öğe Comparative study of functional properties of eight walnut (Juglans regia L.) genotypes(Soc Brasileira Ciencia Tecnologia Alimentos, 2017) Cerit, İnci; Sarıçam, Ayşe; Demirkol, Omca; Ünver, Hülya; Sakar, Ebru; Coşansu, SerapEight walnut (Juglans regia L.) genotypes were assessed for the glutathione (GSH) and total phenolic contents (TPC), as well as their antioxidant capacities. The GSH contents were between 1.1 and 7.2 mg 100 g(-1). The TPC contents ranged from 33 to 50.3 mg GAE g(-1), while the ferric ion reducing antioxidant power (FRAP) values ranged from 156 to 302 mg FeSO4 g(-1). A strong correlation (r(2) = 0.8258) was determined between the FRAP values and the TPC. Cupric ion reducing antioxidant capacity (CUPRAC) values ranged from 13 to 35 mg trolox g(-1). The correlation between the CUPRAC values and the GSH contents was weak (r(2) = 0.2069). The walnut extracts (0.2 mg mL(-1) concentration) showed 16.2-40% DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity. A strong correlation between DPPH-TPC was determined (r(2)= 0.8538), and it was concluded that the TPC makes the most important contribution to the antioxidant capacity of the walnut. Additionally, the walnut may be appraised, in view of its nutritional value and health benefits, by considering the GSH content revealed in the current study.Öğe Determination of Phenological Properties and Effective Heat Summation Requirements of Some Apples Varieties in Ankara (Kalecik) Conditions(2017) Ünver, HülyaIn this study, the phenological characteristics of some apple varieties grown in the Kalecik district of Ankara province, the number of days between effective heat summation requirements, and phenological phases were determined. The research was conducted on Royal Gala, Granny Smith, Jersey Mac, Spur Golden and Red Chief apple varieties. The threshold temperature of 10C was accepted as the temperature value for the determination of the heat summation requirements of the varieties. The effective heat summation above 10C during the growing season of varieties was determined as 819.1- 1986.3C days in 2015 and 865.1-2031.2C days in 2016. The number of days from full bloom to harvest was 85-75 days in 2015 and 132-219 days in 2016.Öğe Molecular characterization of ancient olive genotypes from Hatay Province in Turkey(2016) Sakar, Ebru; Ünver, HülyaTurkeys average share of world olive production is between 7% and 10% and the country is the fourth biggest table olive and olive oil producer in the world. More than fifty olive cultivars have been commercialized in Turkey and there are numerous olive genotypes in different olive-growing regions in Turkey that differ from each other in terms of leaf, flower, and fruit characteristics. The aim of the present study was to identify the 40 most widely grown olive genotypes in Hatay Province in Turkey using microsatellite or simple sequence repeat (SSR) markers. Ten SSR loci were selected and used to identify olive genotypes/cultivars. The number of alleles per locus was found to be between 4 (UDO4 and DCA13) and 16 (DCA9), indicating high polymorphism among olive germplasms. We did not determine identical cultivars in SSR analysis. Samandag2 and Dörtyol7 (0.85), Samandag7 and Saurani (0.75), Payas kalesi and Sarı ulak (0.75), and Yayladag4 and Samandag3 (0.70) genotype pairs showed higher similarity while Yayladag1 and Samandag8 (0.15), Reyhanlı1 and Yayladag6 (0.15), and Samandag8 and Hassa5 (0.15) were found to be the most genetically divergent genotypes.Öğe Şırnak İli Zeytin Gen Kaynaklarının Morfolojik, Pomolojik Özellikleri İle Yağ Asidi Kompozisyonlarının Belirlenmesi(2017) Sakar, Ebru; Ünver, Hülya; Ulaş, Mehmet; Ercişli, SezaiÜlkemiz Güneydoğu Anadolu Bölgesi'nde yer alan Şırnak ilindeki zeytin gen kaynaklarının oluşturduğu populasyon içerisinden üstün nitelikli olanları belirlemek amacıyla gerçekleştirilen çalışmada, 34 genotipten sürgün, yaprak ve meyve örnekleri alınmıştır. Belirlenen genotiplerde ağaç, meyve ve yaprak özellikleri ile toplam yağ ve yağ asitleri kompozisyonları incelenmiştir. Şırnak ili genotiplerinin çoğunlukla dik ve yarı dik taç yapısında ve tiplerin çoğunluğunun eliptik uzun şekilli yaprağa sahip oldukları görülmüştür. İncelenen tiplerde meyve ağırlığı 0.70 g (Deran5)-4.20 g (Serekani) ve meyve şekli tiplerin çoğunluğunda eliptik olarak belirlenmiştir. Toplam yağ oranı % 2.0 ile % 8.8 arasında bulunmuştur. Yağ asitleri miktarları, palmitik asit %12.57 (Karkamış3)-%19.82 (Oğuzeli1), stearik asit %2.31 (Islahiye1)%4.23 (Araban2), oleik asit %58.68 (Oğuzeli3)-%72.86 (Karkamış3), linoleik asit %5.10 (Araban1)-%21.06 (Oğuzeli3) ve linolenik asit %0.73 (Oğuzeli1)-%1.71 (Nizip9, Nizip10) olarak belirlenmiştirÖğe KOCAELİ YÖRESİ CEVİZLERİNİN (Juglans regia L.) ÖN SELEKSİYONU(2017) Ünver, Hülya; Durul, Melekber SülüşoğluKocaeli yöresinde tohumdan yetişen ceviz ağaçlarının oluşturduğu populasyon içerisinden üstün özellikli tipleri seçmek amacıyla ön seleksiyon çalışması gerçekleştirilmiştir. Bu amaçla ceviz potansiyeli bakımından önemli yerleşim birimleri gezilmiş, yetiştiricinin verdiği ön bilgiler ve seleksiyon kriterleri gözönünde bulundurularak 148 ağaçtan meyve örnekleri alınmıştır. Tiplerde önemli meyve özellikleri incelenmiştir. Araştırma sonucunda incelenen tiplerde meyve ağırlığı 16.96-6.33 g, iç ağırlığı 9.02-2.03 g, iç oranı %67.41-%29.93, kabuk kalınlığı 2.30-1.19 mm arasında belirlenmiştir. Tipler dolu ve sağlam iç oranı yönünden iyi durumda bulunmuş olup 129 tip %100 oranında dolu ve sağlam iç vermiş, 19 tipte boş meyveye rastlanılmıştır
