Yazar "Turumtay, Halbay" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim
    Öğe
    beta-lactamase genes in carbapenem resistance Acinetobacter baumannii isolates from a Turkish university hospital
    (J Infection Developing Countries, 2019) Coşkun, Umut Safiye Say; Çalışkan, Emel; Çiçek, Ayşegül Çopur; Turumtay, Halbay; Sandallı, Cemal
    Introduction: The spread of Acinetobacter baumannii, resistant to most of the available antimicrobial agents, is a serious health problem. The high rate of carbapenem resistance among Acinetobacter baumannii isolates is considered as a threat to public health. In this study, we aimed to determine the antibiotic resistance and related genes in carbapenem-resistant Acinetobacter baumannii isolates. Methodology: Ninety six isolates of A. baumannii were included. Antimicrobial susceptibility was performed by Phoenix Automated System and disk diffusion method. Carbapenem resistane was characterized by scrneeing of resistance genes such as bla(TEM), bla(SHV), bla(CTX-M1-2), bla(PER), bla(VEB), bla(KPC), bla(GES), bla(NDM), bla(VIM), bla(IMP) and bla(OXA23-24-51-58) using multiplex polymerase chain reaction. Results: Resistance for the levofloxacin, gentamicin, amikacin, and tigecycline were determined as 96.9%, 93.7%, 72.9% and 45.8% respectively. Colistin was the only susceptible antibiotic against all clinical isolates. All isolates were defined as multidrug resistance and of these, 31.2% were extensively drug-resistant (sensitive only to colistin). B/aoxA-51 and b/aoxA-23 genes were detected in 100% strains while bla(TEM) was found in only 2% strains. There was no amplification for the bla(SHV), bla(CTX-M1-2), bla(PER), bla(VEB), bla(KPC), bla(GES) bla(NDM), bla(VIM), bla(IMP) and bla(OXA24-58) genes. Conclusions: The high frequency of bla(OXA-23) and low frequency of bla(TEM) gene was observed that indicate prevalence of a variety of A. baumannii strains. The rates of resistance genes vary from region to region. Studies are required for the prevention and control of A. baumannii infection and to formulate the strategies of antibiotic usage.
  • Küçük Resim Yok
    Öğe
    Impact of magnetic field on the translocation of iron oxide nanoparticles (Fe3O4) in barley seedlings (Hordeum vulgare L.)
    (Springer Heidelberg, 2023) Tombuloglu, Huseyin; Ercan, Ismail; Alqahtani, Noha; Alotaibi, Bayan; Bamhrez, Muruj; Alshumrani, Raghdah; Turumtay, Halbay
    The effect and contribution of an external magnetic field (MF) on the uptake and translocation of nanoparticles (NPs) in plants have been investigated in this study. Barley was treated with iron oxide NPs (Fe3O4, 500 mg/L, 50-100 nm) and grown under various MF strengths (20, 42, 125, and 250 mT). The root-to-shoot translocation of NPs was assessed using a vibrating sample magnetometer (VSM) and inductively coupled plasma optical emission spectrometry (ICP-OES). Additionally, plant phenological parameters, such as germination, protein and chlorophyll content, and photosynthetic and nutritional status, were examined. The results demonstrated that the external MF significantly enhances the uptake of NPs through the roots. The uptake was higher at lower MF strengths (20 and 42 mT) than at higher MF strengths (125 and 250 mT). The root and shoot iron (Fe) contents were approximately 2.5-3-fold higher in the 250 mT application compared to the control. Furthermore, the MF treatments significantly increased micro-elements such as Mn, Zn, Cu, Mo, and B (P < 0.005). This effect could be attributed to the disruption of cell membranes at the root tip cells caused by both the MF and NPs. Moreover, the MF treatments improved germination rates by 28%, total protein content, and photosynthetic parameters. These findings show that magnetic field application helps the effective transport of magnetic NPs, which could be essential for NPs-mediated drug delivery, plant nutrition, and genetic transformation applications.