Yazar "Tombuloglu, Huseyin" seçeneğine göre listele

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    Effects of foliar iron oxide nanoparticles (Fe 3 O 4 ) application on photosynthetic parameters, distribution of mineral elements, magnetic behaviour, and photosynthetic genes in tomato ( Solanum lycopersicum var. cerasiforme) plants
    (Elsevier France-Editions Scientifiques Medicales Elsevier, 2024) Tombuloglu, Guzin; Tombuloglu, Huseyin; Slimani, Yassine; Almessiere, Munirah A.; Baykal, Abdulhadi; Bostancioglu, Safiye Merve; Kirat, Gokhan
    This study aims to examine the effect of foliar magnetic iron oxide (Fe 3 O 4 ) nanoparticles (IONP) application on the physiology, photosynthetic parameters, magnetic character, and mineral element distribution of cherry tomatoes ( Solanum lycopersicum var. cerasiforme ). The IONP suspension (500 mg L -1 ) was sprayed once (S1), twice (S2), thrice (S3), and four times (S4) a week on seedlings. Upon 21 days of the treatments, photosynthetic parameters (chlorophyll, carotenoids, photosynthetic yield, electron transport rate) were elucidated. Inductivelycoupled plasma -optical emission spectrometer (ICP-OES) and vibrating sample magnetometer (VSM) were used to determine the mineral elements and abundance of magnetic power in the seedlings. In addition, the RTqPCR method was performed to quantify the expressions of photosystem-related ( PsaC , PsbP6 , and PsbQ ) and ferritin-coding ( Fer-1 and Fer-2 ) genes. Results revealed that the physiological and photosynthetic indices were improved upon S1 treatment. The optimal dosage of IONP spraying enhances chlorophyll, carotenoid, electron transport rate (ETR), and effective photochemical quantum yield of photosystem II (Y(II)) but substantially diminishes non -photochemical quenching (NPQ). However, frequent IONP applications (S2, S3, and S4) caused growth retardation and suppressed the photosynthetic parameters, suggesting a toxic effect of IONP in recurrent treatments. Fer-1 and Fer-2 expressions were strikingly increased by IONP applications, suggesting an attempt to neutralize the excess amount of Fe ions by ferritin. Nevertheless, frequent IONP treatment fluctuated the mineral distribution and caused growth inhibition. Although low -repeat foliar applications of IONP (S1 in this study) may help improve plant growth, consecutive applications (S2, S3, and S4) should be avoided.
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    Formulation of Manganese Zinc Spinel Ferrite (Mn0.5Zn0.5Fe2O4) Nanoparticles for the Growth Promotion of Plants
    (Springer Int Publ Ag, 2023) Tombuloglu, Huseyin; Alsaeed, Moneerah; Slimani, Yassine; Demir-Korkmaz, Ayse; Tombuloglu, Guzin; Sozeri, Huseyin; Almessiere, Munirah A.
    This research investigates the uptake and potential contribution of engineered manganese-zinc (MnZn) spinel ferrite nanoparticles (Mn0.5Zn0.5Fe2O4 NPs) to the growth performance of pumpkin (Cucurbita maxima L.). For this purpose, MnZn spinel ferrites were synthesized, and their structural, microstructural, and magnetic properties were determined. NPs (50, 100, 200, and 400 mg L-1) were applied to pumpkin seedlings in a hydroponic system for a week, and the root, stem, and leaf tissues were screened for NPs-uptake by using X-ray powder diffraction (XRD), vibrating sample magnetometry (VSM), and X-ray fluorescence (XRF). Besides, the effect of NPs treatments on some phenological parameters such as pigmentation, photosynthetic efficiency, and biomass was determined. The results showed that MnZn spinel ferrite treatment significantly increased Mn, Zn, and Fe content in the root, stem, and leaf. The Fe, Zn, and Mn content in the leaves increased by approximately 48, 67, and 20 times, respectively, when 400 mg L-1 was applied. Similarly, the magnitude of magnetization of root, stem, and leaf specimens confirmed the incorporation and translocation of magnetic NPs into plant tissues. Besides, the photosynthetic efficiency, pigmentation, and fresh weight were significantly enhanced, suggesting growth improvement by engineered NPs. NP concentrations for the most efficient plant growth were determined as 100 and 200 mg L-1. However, higher NP concentrations suppressed the growth due to the migration/translocation of excess NPs. These findings revealed the potential of engineered MnZn spinel ferrite NPs as nano-fertilizers to provide essential micronutrients, Mn, Zn, and Fe in this study. However, environmental concerns must be considered when using NPs at large scales.
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    Impact of magnetic field on the translocation of iron oxide nanoparticles (Fe3O4) in barley seedlings (Hordeum vulgare L.)
    (Springer Heidelberg, 2023) Tombuloglu, Huseyin; Ercan, Ismail; Alqahtani, Noha; Alotaibi, Bayan; Bamhrez, Muruj; Alshumrani, Raghdah; Turumtay, Halbay
    The effect and contribution of an external magnetic field (MF) on the uptake and translocation of nanoparticles (NPs) in plants have been investigated in this study. Barley was treated with iron oxide NPs (Fe3O4, 500 mg/L, 50-100 nm) and grown under various MF strengths (20, 42, 125, and 250 mT). The root-to-shoot translocation of NPs was assessed using a vibrating sample magnetometer (VSM) and inductively coupled plasma optical emission spectrometry (ICP-OES). Additionally, plant phenological parameters, such as germination, protein and chlorophyll content, and photosynthetic and nutritional status, were examined. The results demonstrated that the external MF significantly enhances the uptake of NPs through the roots. The uptake was higher at lower MF strengths (20 and 42 mT) than at higher MF strengths (125 and 250 mT). The root and shoot iron (Fe) contents were approximately 2.5-3-fold higher in the 250 mT application compared to the control. Furthermore, the MF treatments significantly increased micro-elements such as Mn, Zn, Cu, Mo, and B (P < 0.005). This effect could be attributed to the disruption of cell membranes at the root tip cells caused by both the MF and NPs. Moreover, the MF treatments improved germination rates by 28%, total protein content, and photosynthetic parameters. These findings show that magnetic field application helps the effective transport of magnetic NPs, which could be essential for NPs-mediated drug delivery, plant nutrition, and genetic transformation applications.
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    Magnetic Behavior and Nutrient Content Analyses of Barley (Hordeum vulgare L.) Tissues upon CoNd0.2Fe1.8O4 Magnetic Nanoparticle Treatment
    (Springer International Publishing Ag, 2020) Tombuloglu, Huseyin; Slimani, Yassine; Alshammari, Thamer; Tombuloglu, Guzin; Almessiere, Munirah; Baykal, Abdulhadi; Demirci, Tuna
    This study investigates (i) in planta uptake and transfer of magnetic nanoparticles (MNPs) in the plant body and (ii) impact of MNPs on plant nutrition. For these purposes, barley (Hordeum vulgare L.) seedlings were subjected by varied MNP doses (125 to 1000 mg L-1 of CoNd0.2Fe1.8O4) for 3 weeks in a hydroponic system. Plant tissues (root and leaf) were analyzed by using vibrating sample magnetometer (VSM) and inductively coupled plasma optical emission spectrometer (ICP-OES) techniques to understand MNPs' uptake and translocation in the plant body, and plant nutrition status as well. Elemental composition and magnetic behavior analyses of plant parts proved that MNPs, sized in 8.4 +/- 0.05 nm, are uptaken by the plant roots and led to an increase in iron (Fe), neodymium (Nd), and cobalt (Co) contents of leaves (p < 0.005). However, compared with the untreated control, the amount of some macro- and micro-elements (K, Ca, Mg, Mn, and P) are declined in the leaf by increased MNP doses (p < 0.05). Root-to-leaf translocation index (%) of the elements were dramatically decreased, except the one for Fe which increased from 25 (control) to 55% in 1000 mg L-1 condition. Accordingly, MNPs are uptaken by the plant roots and transferred to the leaves. However, it suppresses the translocation of essential nutrients. This finding shows that MNPs used in this study is detrimental for plant mineral nutrition. Besides, the VSM technique coupled with ICP-OES enables to track MNPs in the plant body.
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    Structural, spectroscopic, dielectric, and magnetic properties of Fe/Cu co-doped hydroxyapatites prepared by a wet-chemical method
    (Elsevier, 2022) Ercan, Ismail; Kaygili, Omer; Kayed, Tarek; Bulut, Niyazi; Tombuloglu, Huseyin; Ince, Turan; Koysal, Oguz
    In this study, the effects of Cu and Fe additives on the structural, dielectric, magnetic, thermal and morphology of hydroxyapatite (HAp) samples were investigated and reported in detail for the first time. The prepared systems were also modeled and examined theoretically. It can be said that both additives affect the thermal behavior of the HAp structure. The addition of Cu affected the morphology of submicron-sized particles with spherical-like shapes with a low degree of agglomeration. Diffuse reflection data revealed that the energy band gap values decreased as Cu was added to the Fe-based HAp structure. The dielectric constant (epsilon') had high values at low frequencies in all samples and decreased with increasing frequency. It could be concluded that Fe/Cu incorporation to hydroxyapatites enhanced its thermal, magnetic, and dielectric properties required for mimicking natural HAps to open a successful venue for medical application in the healing regeneration of bone.
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    Uptake and bioaccumulation of iron oxide nanoparticles (Fe3O4) in barley (Hordeum vulgare L.): effect of particle-size
    (Springer Heidelberg, 2024) Tombuloglu, Guzin; Aldahnem, Anwar; Tombuloglu, Huseyin; Slimani, Yassine; Akhtar, Sultan; Hakeem, Khalid Rehman; Almessiere, Munirah A.
    Root-to-shoot translocation of nanoparticles (NPs) is a matter of interest due to their possible unprecedented effects on biota. Properties of NPs, such as structure, surface charge or coating, and size, determine their uptake by cells. This study investigates the size effect of iron oxide (Fe3O4) NPs on plant uptake, translocation, and physiology. For this purpose, Fe3O4 NPs having about 10 and 100 nm in average sizes (namely NP10 and NP100) were hydroponically subjected to barley (Hordeum vulgare L.) in different doses (50, 100, and 200 mg/L) at germination (5 days) and seedling (3 weeks) stages. Results revealed that particle size does not significantly influence the seedlings' growth but improves germination. The iron content in root and leaf tissues gradually increased with increasing NP10 and NP100 concentrations, revealing their root-to-shoot translocation. This result was confirmed by vibrating sample magnetometry analysis, where the magnetic signals increased with increasing NP doses. The translocation of NPs enhanced chlorophyll and carotenoid contents, suggesting their contribution to plant pigmentation. On the other hand, catalase activity and H2O2 production were higher in NP10-treated roots compared to NP100-treated ones. Besides, confocal microscopy revealed that NP10 leads to cell membrane damages. These findings showed that Fe3O4 NPs were efficiently taken up by the roots and transported to the leaves regardless of the size factor. However, small-sized Fe3O4 NPs may be more reactive due to their size properties and may cause cell stress and membrane damage. This study may help us better understand the size effect of NPs in nanoparticle-plant interaction.