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    Crocin Protects Mice Pancreatic Islets from Oxidative Stress Induced by Methylglyoxal and Increases Insulin Secretion
    (Düzce Üniversitesi, 2023) Radmehr, Vahid; Ahangarpour, Akram; Haroonı, Elnaz; Noeı Razlıqı, Reza
    Aim: Islets of Langerhans are more sensitive to oxidative damage because of their low antioxidant capacity. In diabetes, methylglyoxal (MG) accumulates in the pancreas. The present study examined the effect of crocin on oxidative stress induced by MG in isolated Langerhans islets from male mice.Material and Methods: Twenty-four male mice weighing 20 to 25 g were prepared. The isolated Langerhans islets were transferred to the culture medium. Oxidative stress was induced through MG administration for 30 min, and then 10, 20, 30, and 40 ?M of crocin was used for 2 h. Samples were divided into seven groups with 2.8, 5.6, and 16.7 mM glucose concentrations: control, MG 300 ?M, MG+glibenclamide 10 ?M, and MG+crocin in four doses of 10, 20, 30, and 40 ?M. At the end, the islet’s insulin, antioxidant levels, and lipid peroxidation were assessed by ELISA and calorimetry methods.Results: Increased levels of malondialdehyde (MDA) in MG groups significantly decreased in 2.8 (p=0.008), 5.6 (p=0.004), and 16.7 (p?0.001) mM glucose concentrations, with administration of 30 and 40 ?M crocin. Total antioxidant capacity (TAC) was reduced in MG groups (p?0.001) and significantly restored in all crocin-treated groups in 2.8, 5.6, and 16.7 mM glucose concentrations. Also, a significant decrease in insulin secretion and content was observed in MG groups of all three glucose concentrations (p?0.001). Crocin at high doses improved these alterations.Conclusion: MG caused oxidative damage and reduced insulin secretion in isolated islets. Crocin improved the antioxidant defense system, diminished MDA, and increased insulin secretion.
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    Protective Effects of Myricitrin and Vitamin E on Nephropathy of Aging Mice Model Induced By D-Galactose
    (2021) Ahangarpour, Akram; Radmehr, Vahid; Omidi, Mina; Khorsandi, Layasadat
    Aim: Aging occurs in cells and tissues due to oxidative stress in physiological conditions. D-galactose (DG) is widely used to cause aging in animal studies. In this study, the renal protective effects of myricitrin and vitamin E in the aging mice model induced by DG was evaluated. Material and Methods: Subcutaneous DG injection was used for induction of the aging model. 72 female mice were randomly divided into six groups: All groups were received DG at 500 mg/kg/d for six weeks. In the last 28 days, the groups treated with myricitrin subcutaneously received 5, 10, and 20 mg/kg/d, and the vitamin E group received 100 mg/kg/d by gavage. Urine and plasma albumin, BUN, creatinine levels, MDA, TAC, and kidney histological changes were evaluated. Results: Plasma albumin was significantly decreased (p=0.001), but a significant increase in urine albumin (p=0.001), BUN (p<0.001), and creatinine (p=0.010) levels was observed in the DG group when compared with the control. Also, a significant increase in MDA levels (p=0.002) along with a significant decrease in TAC (p=0.012) was observed. Histopathological changes such as congestion of erythrocytes (p<0.001), infiltration of inflammatory cells (p<0.001), and proximal tubule cell damage (p=0.004) significantly increased, while glomerulus diameter significantly decreased (p=0.038) in comparison to the control. Administration of myricitrin and vitamin E showed a significant ameliorative effect on all studied variables. Conclusion: The improvement effects of myricitrin on DG-induced kidney damage was approximately equivalent to vitamin E. Myricitrin and vitamin E could have beneficial effects on the nephropathy of aging model