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Öğe Analysis of HPV Genotypes and Liquid-Based Cervical Cytology: Results from a Tertiary Academic Center in Northwestern Turkey(Natl Inst Infectious Diseases, 2021) Caliskan, Emel; Coskun, Sinem Kantarcioglu; Ozturk, Cihadiye Elif; Cangur, Sengul; Onail, BinnurHigh-risk human papilloma virus (hrHPV) plays an important role in cervical cancer. The aim of this study was to investigate the distribution of HPV genotypes in the region and to correlate it with liquid-based-cytology (LBC) and colposcopic biopsy results. Furthermore, the potential relationship between HPV infections and bacterial vaginosis (BV) was investigated. HPV genotypes were determined using real-time PCR. LBC, biopsies, and BV examinations were performed by the Pathology and Cytology. Consecutive cervical specimens of 409 women who underwent both cytology and HPV-DNA tests were included in the study. A total of 172 (42.1%) patients were positive for HPV-DNA; of these, 107 (26.2%) had hrHPV. The most common HPV genotypes were HPV 59, 16, 33, 52, and 51, at 16.6%, 15.9%, 13.4%, 13.4%, and 8.9%, respectively. Epithelial cell abnormality was detected in 11.5% of LBC test results. The genotypes of HPV 33, 56, 66, and 68 were found at a higher rate in patients with epithelial cell abnormalities than in those with no detected abnormalities. Bacterial vaginosis was found in 24 patients (5.9%). HPV-DNA positivity was observed to be statistically higher in patients with BV than in those without BV.Öğe Can the Gut Microbiota Serve as a Guide to the Diagnosis and Treatment of Childhood Epilepsy?(Elsevier Science Inc, 2023) Turay, Sevim; Cangur, Sengul; Kahraman, Gozde; Kayabasi, Eda; Cetiner, Omer Faruk; Aydin, Burak; Ozturk, Cihadiye ElifBackground: To investigate the activity of the gut-brain axis in the pathogenesis of childhood epilepsy and to define biomarkers capable of assisting with determining new strategies in that context. Methods: Twenty children with epilepsy of unknown etiology and seven healthy controls in the same age group were included in the study. The groups were compared using a questionnaire. Stool samples were stored in tubes containing DNA/RNA Shield (Zymo Research) with a sterile swab. Sequencing was carried out using the MiSeq System (Illumina). The 16S rRNA sequencing of samples using nextgeneration sequencing involved V4 variable region polymerase chain reaction amplification concluded by 2 x 250-bp paired-end sequencing of amplicons and at least 50,000 reads (>Q30) per sample. DNA sequences were classified at the genus level using the Kraken program. Bioinformatics and statistical analysis were then performed.Results: Individuals' gut microbiota relative abundance values differed between the groups at the genus, order, class, family, and phylum levels. Flavihumibacter, Niabella, Anoxybacillus, Brevundimonas, Devosia, and Delftia were seen only in the control group, whereas Megamonas and Coriobacterium were observed only in the epilepsy group. The linear discriminant analysis effect size method identified 33 taxa as important in differentiating the groups. Conclusions: We think that bacterial varieties (such as Megamonas and Coriobacterium) that differ be-tween the two groups can be employed as useful biomarkers in the diagnosis and follow-up of epileptic patients. We also predict that, in addition to epilepsy treatment protocols, the restoration of eubiotic microbiota may increase the success of treatment.& COPY; 2023 Elsevier Inc. All rights reserved.Öğe The evaluation of both the expression and serum protein levels of Caspase-3 gene in patients with different degrees of SARS-CoV2 infection(Wiley, 2021) Gulhan, Pinar Yildiz; Eroz, Recep; Ataoglu, Ozlem; Ince, Nevin; Davran, Fatih; Ozturk, Cihadiye Elif; Balbay, Oner AbidinTo evaluate the effects of Caspase-3 (CASP3) gene expression and serum levels on preventing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. A total of 41 individuals (male: 21; female: 20) with SARS-CoV-2 infection were included in the current study. Hemograms were examined from patient blood samples, and CASP3 gene expression levels were detected. Also, human CASP3 levels were determined from the serum samples of patients. The mean age of patients was 56.220 +/- 18.937 years. Significant differences were detected among all groups for CASP3 2-Delta Delta Ct (p = 0.014) and CASP3 concentration (p = 0.024). The relationship between CASP3 2-Delta Delta Ct levels and hemoglobin (p = 0.023), between CASP3 2-Delta Delta Ct levels and C-reactive protein (CRP) (p = 0.001), between CASP3 2-Delta Delta Ct levels and ferritin (p = 0.003), between CASP3 2-Delta Delta Ctlevels and lactate dehydrogenase (p = 0.001), and between CASP3 2-Delta Delta Ct levels and SpO(2) (p = 0.006) were statistically significant. Also, the relationship between CASP3 concentration levels and SpO(2) was statistically significant (p < 0.046). The CASP3 gene and/or its products have an important function to prevent injury caused by SARS-CoV-2 infection. They play crucial roles in maintaining cellular homeostasis and viability. Perhaps CASP3 levels may provide information about the severity of the disease.Öğe Identification of the Gram Positive Bacterial Sepsis Agent with Rapid Genotype Test(Duzce Univ, Fac Medicine, 2021) Erdogan, Ziya; Ozturk, Cihadiye Elif; Cangur, Sengul; Caliskan, Emel; Oksuz, Sukru; Karamurat, Zeynep DilaraObjective: An irreversible process begins when a systemic infection causes sepsis. Therefore, rapid identification of the agent bacteria in sepsis and its antibiotic resistance is crucially important. In this study, it was aimed to investigate the efficiency of rapid genotype test in detecting sepsis agent Gram positive bacteria and important antibiotic resistance. Methods: 2132 blood culture samples sent to the laboratory were examined with an automatic blood culture system (BACTEC, BD, USA) between 2018-2019. Blood culture bottles sent to the laboratory were Growing bacteria was identificated by VITEK (bioMerieux, France) automated bacteria identification / antibiotic susceptibility system. In addition, bacterial species and mecA, vanA, vanB, vanC1, vanC2 / C3 genes in blood cultures with Gram positive bacterial growth were also determined by the Genotype (R) BC Gram-positive (Hain Lifesience, Germany) test. Results: 72 patients with gram-positive bacteria growth in two or more blood culture bottles were included in the study. In 44 of the samples (61%) the same bacterial species were detected with conventional method (bacteria culture) and BC Gram positive test. In 28 of the samples (39%) differences were detected between results of methods regarding bacterial species name or vancomycin/methicillin resistance rate. Although single agent was isolated with culture method in all of the samples, multiple agents were detected in eight samples with rapid genotype test. Also, it was found that in mecA positive samples, ciprofloxacin resistance was higher than mecA negative ones. Conclusions: In the study, it was observed that BC Gram positive test could correctly identify sepsis agent bacteria and their resistance genes within 4-5 hours.Öğe Monitoring of antibody levels in healthcare workers after inactivated coronavirus disease 19 vaccination(Assoc Medica Brasileira, 2023) Caliskan, Emel; Ozturk, Cihadiye Elif; Oksuz, Sukru; Ince, Nevin; Yekenkurul, Dilek; Kahraman, Gozde; Duran, PelinOBJECTIVE: Because of the coronavirus disease 19 pandemic, studies on vaccination are being conducted in our country as well as across the world. In this study, the antibody levels in healthcare workers vaccinated with two doses of inactivated vaccine and the factors affecting these levels were investigated. METHODS: Randomly selected volunteers from healthcare workers, who had been vaccinated with two doses of inactivated vaccine in January to February 2021, were included in the study. Blood samples were drawn twice, 1 month and 6 months after the second dose vaccine (CoronaVac:Sinovac Life Science Co, Ltd, Beijing, China). The antibody levels were determined by the chemiluminescence microparticle immunoassay method using kits for quantitative detection of immunoglobulin class G antibodies to severe acute respiratory syndrome coronavirus 2. RESULTS: The mean antibody levels of 129 volunteers were 1232.5 (min: 103 to max: 7151) AU/mL in the first month and 403.5 (min: 23 to max: 4963) AU/mL in the sixth month. According to the survey results, 91 (71%) volunteers had not been diagnosed with coronavirus disease 19 before vaccination. The antibody levels 1 month and 6 months after the second dose of vaccination were significantly higher in those who had been diagnosed with coronavirus disease 19 before vaccination than in those who had not. It was found that age, gender, fast food, or healthy nutrition had no effect on antibody levels. CONCLUSION: Vaccines are very important both to protect against coronavirus disease 19 and to experience only a mild form of the disease. Immunoglobulin class G levels formed after vaccination may be affected by many factors and may decrease over time.Öğe A Visceral Leishmaniasis Case from the Black Sea Region: Skin Lesions and Leishmania donovani Amastigotes in the Bone Marrow(Galenos Publ House, 2024) Onec, Birguel; Ozturk, Cihadiye Elif; Yazici, Ayten[No abstract available]
