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  • Küçük Resim
    Öğe
    Does capsaicin have therapeutic benefits in human colon adenocarcinoma? Selection of the most reliable dose via AgNOR
    (2020) Nisari, Mustafa; Eröz, Recep
    Background/aim: To determine the effect of different doses of capsaicin on AgNOR protein synthesis in human colon adenocarcinomaderivate from colon cancer (Caco-2 cell).Materials and methods: In this experimental study, after the cultured of Caco-2 cell line, the cells are divided into 4 groups as controland different capsaicin exposed doses (25uµ, 50uµ, and 75uµ). Mean AgNOR number and total AgNOR area/nuclear area (TAA/NA)were calculated.Results: A significant differences were detected between control and capsaicin (50uµ) (P = 0.001), between control and capsaicin (75uµ)(P = 0.000), between capsaicin (25uµ) and capsaicin (50uµ) (P = 0.001) and between capsaicin (25uµ) and capsaicin (75uµ) (P = 0.000)for TAA/NA. Also, there were significant differences between control and capsaicin (50uµ) (P = 0.001), between control and capsaicin(75uµ) (P = 0.000), between capsaicin (25uµ) and capsaicin (50uµ) (P = 0.000) and between capsaicin (25uµ) and capsaicin (75uµ) (P= 0.000) for mean AgNOR number.Conclusion: A certain amount of capsaicin has a protective effect against colon adenocarcinoma and the dose concentrations areimportant for the most reliable treatment.
  • Küçük Resim
    Öğe
    May argyrophilic nucleolar organizing region-associated protein synthesis be used for selecting the most reliable dose of drugs such as rhamnetin in cancer treatments?
    (Comenius Univ, 2016) Ertekin, Tolga; Bozkurt, Özlem; Eröz, Recep; Nisari, Mehtap; Bircan, Duygu; Nisari, Mustafa; Unur, Erdoğan
    BACKGROUND: Rhamnetin is a flavonoid that has antioxidant, anti-inflammatory and anti-cancer effects. Nucleolar-organizing regions are the ribosomal genes region. We aimed to identify whether rhamnetin has an effect on cell proliferation and whether AgNOR proteins may be used for the detection of therapeutic benefits of the drugs and new metabolites, which have the potential of being used for cancer treatments. METHODS: Twenty-four mice with Ehrlich's ascites carcinoma (EAC) were randomly assigned to three main groups as positive control, and groups 2 and 3 treated intraperitoneally with rhamnetin (100 mu g/kg and 200 mu g/kg, respectively). All the animals were sacrificed on day 16, 24 h after the last dose; the tumors, which developed at the site of injection were removed. Then, mean AgNOR number and total AgNOR area/nuclear area (TAA/NA) were detected for each mouse. RESULTS: Significant differences were detected among all groups for mean AgNOR number (p = 0.000) and TAA/NA ratio (p = 0.000). While the difference between positive control and Rhamnetin (100 mu g/kg) group was not significant (p = 0.387), there are signifi cant differences between positive control and Rhamnetin (200 mu g/kg) group (p = 0.000) and between Rhamnetin (100 mu g/kg) and Rhamnetin (200 mu g/kg) groups (p = 0.000) for TAA/NA ratio. CONCLUSION: Rhamnetin has an important role in preventing cancer formation. Our study showed that mean AgNOR numbers and TAA/NA values may be used also as biomarkers for evaluating the success rate of the performed therapeutic strategy and accurate dose selection for the management of the disease (Tab. 3, Fig. 3, Ref. 45). Text in PDF www.elis.sk.