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Determination of Allelic Variation of Karahan-99 × BW3 Bread Wheat Population F4 Individuals Using DNA Markers
(Türk Ziraat Yüksek Mühendisleri Birliği, 2023) Yüce, İlker; Topsakal, Münire; Tekin, Ali; Tekin, İmren Çöken; Demirezen, Ayşe Nur; Şenay, Ali; Korucu, Burakhan
Wheat is the main nutrient that mankind has used since settled life. Plant breeders are making great efforts to increase the yield of wheat plant in parallel with the increasing world population. In this study, 22 F4 lines obtained as a result of crossing Karahan-99 bread wheat variety with BW3 wheat genotypes were characterized with 11 functional DNA markers belong to some agronomic traits. Of the 11 markers used in the study, seven worked (Xgwm18, Xwgp118, Xgwm131, Xgwm47, Sun479, Sun209 and UHW89) in Karahan-99 × BW3 Bread Wheat Population. While the highest number of alleles was obtained from the Sun479 marker (18 bands), the lowest band number was obtained from the markers UHW89 and Xgwmn131 (1 band). The mean number of alleles was 6.71, while the mean polymorphism information content (PIC) was 0.99. Xgwm131 marker encoding Yr39 yellow rust allele was interrogated at Karahan-99_M6_72-10/BW3_F4_3 genotype, while Xgwm18 marker amplified Yr26 stripe rust resistant gene at Karahan-99_M6_72-10/BW3_F4_22.