Yazar "Karkucak, Mutlu" seçeneğine göre listele
Listeleniyor 1 - 4 / 4
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Does MBL2 codon 54 polymorphism play a role in the pathogenesis of psoriasis?(Wiley-Blackwell, 2014) Turan, Hakan; Karkucak, Mutlu; Yakut, Tahsin; Özşahin, Mustafa; Gürlevik, Zehra; Yanık, Mehmet Emin; Yaykaşlı, Kürşat OğuzBackground Psoriasis is a T cell-mediated immune disease in which various cytokines, primarily tumor necrosis factor- (TNF-), are complexly involved. Mannose-binding lectin (MBL) gene polymorphisms decrease MBL serum levels, thereby increasing the synthesis of proinflammatory cytokines such as TNF-. Objectives This trial was designed to evaluate the role of the MBL2 codon 54 polymorphism in the pathogenesis of psoriasis. Methods Fifty patients diagnosed with psoriasis vulgaris and 53 healthy subjects were included in the trial. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was applied to determine the MBL2 codon 54 polymorphism. Genotypes were determined according to the bands formed in agarose electrophoresis gels. For the statistical analysis, the level of significance was set at P<0.05. Results A total of 33 (66.0%) of the 50 psoriasis patients were detected to have A/A genotype and 17 (34.0%) had B/B genotype. Of the control subjects, 44 (83.0%) had A/A genotype and nine (17.0%) had B/B genotype. There was a statistically significant difference between the groups (P=0.047). The analysis of allele frequencies revealed A allele prevalences to be 79 (79.0%) and 95 (89.6%), and B allele prevalences to be 21 (21.0%) and 11 (10.4%), in the patient and control groups, respectively. A statistically significant difference between allele frequencies was detected (P=0.031). Conclusions This study suggests that the MBL2 codon 54 polymorphism may have an association with psoriasis in the Turkish population.Öğe Glutathione S-Transferase M1 and T1 Gene Polymorphisms in Patients with Chronic Plaque-Type Psoriasis: A Case-Control Study(Karger, 2016) Solak, Berna; Karkucak, Mutlu; Turan, Hakan; Ocakoğlu, Gökhan; Sağ, Şebnem Özemri; Uslu, Esma; Erdem, TeomanObjective: To determine the role of glutathione S-transferase (GST) isoenzyme polymorphisms as susceptibility factors in patients with psoriasis in a Turkish cohort. Subjects and Methods: In this case-control study, 105 patients with plaque-type psoriasis and 102 healthy controls were recruited from the dermatology outpatient clinics of two university hospitals. Genomic DNA was extracted from whole blood using a DZ DNA isolation kit. Multiplex PCR was used to determine GSTM1 and GSTT1 polymorphisms in the isolated DNAs. Results: Of the 150 patients with psoriasis, 83 (79%) were identified with the GSTT1 genotype and 22 (21%) with the null genotype. Of the 102 patients in the control group, 69 (67.6%) subjects were identified with the GSTT1 genotype and 33 (32.4%) with the null genotype. There was no significant difference between the patient and control groups (p = 0.063). Regarding the GSTM1 polymorphism, 54 (51.4%) patients were identified with this genotype and 51 (48.6%) with the null genotype; in the control group, 50 (49%) were identified with this genotype and 52 (51%) with the null genotype. Again there was no statistically significant difference between the groups (p = 0.957). Conclusion: In this Turkish cohort of patients with psoriasis, neither GSTT1 nor GSTM1 polymorphisms were associated with disease susceptibility. Larger studies with a wider range of GST isoenzyme are needed. (C) 2015 S. Karger AG, BaselÖğe Investigation of Monnose-Binding Lectin gene Polymorphism in Patients with Erythema Multiforme, Stevens-Johnson Syndrome and Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis Overlap Syndrome(Aves Yayincilik, 2012) Karkucak, Mutlu; Bülbül, Emel Başkan; Turan, Hakan; Yakut, Tahsin; Toka, Sevil; Sarıcaoğlu, HayriyeObjective: Monnose-Binding lectin (MBL) appears to play an important role in the immune system. The genetic polymorphisms in the MBL2 gene can result in a reduction of serum levels, leading to a predisposition to recurrent infection. The aim of this study is to investigate the influence of a polymorphism in codon 54 of the MBL2 gene on the susceptibility to Erythema Multiforme, Stevens-Johnson Syndrome and Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis Overlap Syndrome (EM, SJS and SJS/TEN overlap syndrome). Material and Methods: Our study included 64 patients who were clinically and/or histopathologically diagnosed with EM, SJS, and SJS/TEN overlap syndrome and 66 healthy control subjects who were genotyped for the MBL2 gene codon 54 polymorphism using the PCR-RFLP method. For all statistical analyses, the level of significance was set at p<0.05. Results: The prevalence of the B allele was 18% in the EM, SJS and SJS/TEN patient groups and 13% in the control group. No significant differences in allele frequencies of any polymorphism were observed between the patient and control groups, although the B allele was more frequent in the patient groups (p=0.328). Conclusion: Our results provide no evidence of a relationship between MBL2 gene codon 54 polymorphism and the susceptibility to EM, SJS and SJS/TEN overlap syndrome. However, these findings should be confirmed in studies with a larger sample size.Öğe MBL2 Gene Polymorphism and Risk of Vitiligo in Turkish Patients(Kamla-Raj Enterprises, 2015) Karkucak, Mutlu; Solak, Berna; Turan, Hakan; Uslu, Esma; Yakut, Tahsin; Aliağaoğlu, Cihangir; Erdem, TeomanMannose-Binding Lectin (MBL) plays an important role in innate immunity. MBL2 gene polymorphisms affect MBL serum levels. Therefore, this increases the risk of infection and may result in predisposition to autoimmune diseases. The aim of this study was to investigate whether there is an association between the MBL2 gene codon 54 (allele B: rs1800450, c. 161G>A; p. 54Gly>Asp) polymorphism and vitiligo in Turkish patients. One hundred and one patients who were diagnosed with vitiligo and 101 control subjects were included in the study. The DNA was analyzed using the Kbioscience Competitive Allele Specific PCR (KASP) technique. MBL2 gene codon 54 polymorphism frequencies were compared between the two groups. In statistical analysis, the level of significance was set at p<0.05. No significant differences in frequencies of the A allele were observed between the patient and control groups. It was observed at similar frequencies in both groups (p=0.890). The results suggest that the MBL2 gene Codon 54 polymorphism is not associated with an increased risk for the development of vitiligo in Turkish patients.