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    First isolation of a probiotic candidate Enterococcus mundtii from Herniaria glabra L. and evaluation of its wound healing activity
    (Oxford Univ Press, 2023) Fidan, Aysenur; Ugras, Serpil
    In this study focused on probiotic properties of bacterium isolated from Herniaria glabra L. is a medicinal plant. The bacterium was isolated from H. glabra, and it was identified using the molecular method as Enterococcus mundtii AF-1 strain. Antibiotic sensitivity tests showed that AF-1 strain was sensitive to streptomycin, tobramycin, gentamicin, imipenem, erythromycin, and ciprofloxacin. The strain exhibited gamma-haemolytic activity. These results show that the strain can be considered safe. The AF-1 strain showed inhibitory activity against some pathogens, including Bacillus subtilis, Klebsiella pneumoniae, and Yersinia pseudotuberculosis. Additionally, AF-1 strain exhibited high tolerance to low pH, pepsin, pancreatin, and bile salts. These properties showed that the strain may survival under the gastrointestinal conditions. The strain showed 40% DPPH free radical scavenging activity. The autoaggregation rate of the strain was 72.46% and the strain exhibited the high coaggregation rate (70.77% with Escherichia coli, and 63.78% with Listeria monocytogenesis). AF-1 strain showed 38.10% adhesion towards n-hexane, and 47.62% adhesion toward chloroform. It has been found to have moderate hydrophobicity. These results demonstrated the beneficial colonization ability of the strain in the gut. Furthermore, it was observed that living cells of AF-1 strain showed healing activity in the artificial wound area. Result of studies, it is seen that AF-1 strain might be excellent a probiotic candidate. In this study focused on probiotic properties of bacterium isolated from Herniaria glabra L. is a medicinal plant.
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    Probiotic potential and wound-healing activity of Pediococcus pentosaceus strain AF2 isolated from Herniaria glabra L. which is traditionally used to make yogurt
    (Springer, 2024) Ugras, Serpil; Fidan, Aysenur; Yoldas, Pinar Agyar
    Probiotics have been a part of our lives for centuries, primarily through fermented foods. They find applications in various fields such as food, healthcare, and agriculture. Nowadays, their utilization is expanding, highlighting the importance of discovering new bacterial strains with probiotic properties suitable for diverse applications. In this study, our aim was to isolate new probiotic bacteria. Herniaria glabra L., a plant traditionally used for yogurt making in some regions and recognized in official medicine in many countries, was chosen as the source for obtaining probiotic bacteria. We conducted bacterial isolation from the plant, molecularly identified the isolated bacteria using 16S rRNA sequencing, characterized their probiotic properties, and assessed their wound-healing effects. As a result of these studies, we identified the bacterium isolated from the plant as Pediococcus pentosaceus strain AF2. We found that the strain AF2 exhibited high resistance to conditions within the gastrointestinal tract. Our reliability analysis showed that the isolate had gamma-hemolytic activity and displayed sensitivity to certain tested antibiotics. At the same time, AF2 did not show gelatinase and DNase activity. We observed that the strain AF2 produced metabolites with inhibitory activity against E. coli, B. subtilis, P. vulgaris, S. typhimurium, P. aeruginosa, K. pneumoniae, E. cloacae, and Y. pseudotuberculosis. The auto-aggregation value of the strain AF2 was calculated at 73.44%. Coaggregation values against E. coli and L. monocytogenes bacteria were determined to be 56.8% and 57.38%, respectively. Finally, we tested the wound-healing effect of the strain AF2 with cell culture studies and found that the strain AF2 promoted wound healing.