Yazar "Ersoy, Mehmet Akif" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim
    Öğe
    Lung Tissue Damage in the Experimental Carbon Tetrachloride Toxicity and Protective Role of Melatonin Hormone: A light Microscopic and Biochemical Study
    (Düzce Üniversitesi, 2012) Gülcen, Burak; Kuş, Murat Abdülgani; Çolakoğlu, Serdar; Ögetürk, Murat; Kuş, İlter; Ersoy, Mehmet Akif; Karaca, Ömür
    In this study, it was aimed to investigate the protective effects of melatonin hormone against tocarbon tetrachloride (CCl4)-induced toxicity in lung tissue. Twenty-four male Wistar-Albinorats were randomly divided into three equal groups. Rats in group I were used as control. Ratsin group II were injected subcutaneously every other day with CCl4. Rats in group III wereinjected subcutaneously every other day with CCl4 and melatonin. At the end of four weeks ofexperimental period, all animals were killed by decapitation and their lungs were removed. Forbiochemical examination, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)enzyme activities and malondialdehyde (MDA) levels were determinated by aspectrophotometer in some of the lung tissue specimens. For light microscopic examination,tissue specimens were embedded in paraffin blocks following routine histological procedures.In lung tissue samples of rats treated with CCl4, SOD and GSH-Px enzyme activities weresignificantly lower and malondialdehyde (MDA) levels were significantly higher than thecontrol group. Microscopically, interstitial pulmonary hemorrhage, leucocytes with polymorphicnuclei and macrophage infiltration were observed in the lung specimens of rats exposed to CCl4alone.There is a statistically significant increase in SOD and GSH-Px enzyme activities, and adecrease in MDA levels in rats treated with CCl4 and melatonin. Histopathological changescaused by CCl4 toxicity were not observed in rats treated by melatonin. It is concluded thatCCl4 creates oxidative damage in lung tissue and melatonin has protective effects against thisCCl4 induced damage.Anahtar kelimeler: Lung, CCl4, oxidative stress, rat
  • Küçük Resim
    Öğe
    Oxidative Tissue Damage in the Prefrontal Cortex of Pinealectomized Rats and Protective Effects of Melatonin Hormone
    (Düzce Üniversitesi, 2012) Kuş, Murat Abdülgani; Çolakoğlu, Serdar; Gülcen, Burak; Kuş, İlter; Sarsılmaz, Mustafa; Ersoy, Mehmet Akif; Karaca, Ömür
    Aim: An attempt was made to investigate the tissue samples of prefrontal cortex frompinealectomized rats and melatonin applied rats following pinealectomy in biochemical basis.Materials and Methods: Twenty-one, male Wistar rats were divided equally into three groups.Rats in Group I were used as control (sham-pinealectomy). Rats in Group II werepinealectomized. Rats in Group III were injected daily with melatonin (1 mg/kg/)intraperitoneally for 3 months following pinealectomy. At the end of the experimental period,all rats were killed by decapitation and the prefrontal cortexes of rats were removed. Superoxidedismutase (SOD), glutathione peroxidase (GSH-Px) enzyme activities and malondialdehyde(MDA) levels were determined at biochemical levels in the prefrontal cortex specimens.Results: The levels of SOD and GSH-Px in the prefrontal cortex tissue were significantlydecreased and MDA levels were significantly increased in pinealectomized rats compared withthose of the controls. However, increased activities of SOD and GSH-Px enzymes and decreasedlevels of MDA were detected in the rats administered melatonin following pinealectomy.Conclusion: As a result of this study, pinealectomy-induced oxidative damage in the prefrontalcortex tissue in rats were prevented by administration of melatonin hormone