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    Effects of Metoprolol on Experimental Spinal Cord Ischemia-Reperfusion Injury in Rats
    (2021) Karahan, Suleyman Caner; Yaman, Serap Özer; Ercin, Mustafa Emre; Hızıroğlu, Sabahattin; Güvercin, Ali Rıza; Yazar, Uğur
    Aim: The aim of this study was to investigate the neuroprotective effect of metoprolol and its efficacy in reducing lipidperoxidation levels in the spinal cord ischemia-reperfusion model in rats.Material and Methods: Twenty (20) Sprague-Dawley female rats weighing between 220 gr and 280 gr were randomlydivided into 3 groups. Only laparotomy was performed in the control group, and the aorta abdominalis was revealed. Inthe groups other than the control group, clip compression was applied to the aorta abdominalis for 45 minutes. Theischemia group was not given any medication. Metoprolol was administered intraperitoneally at 0.5 mg/kg to themetoprolol group. Motor examination was made according to Tarlov scale at the 1st and 24th hours and then, spinalcords of all rat models were removed. Spinal cord tissue samples were collected for histopathological examination andfor determining malondialdehyde (MDA) level. All rats were sacrificed by draining blood after their motorexaminations.Results: According to motor examination findings at the 1st and 24th hours, metoprolol resulted in a statisticallysignificant improvement in recovery (p=0.045). Histopathological examinations revealed that metoprolol contributed toneurological recovery by reducing neuronal necrosis. MDA levels, which is an indicator of lipid peroxidation, weresignificantly lower in the metoprolol group when compared to the ischemia group (p=0.001).Conclusion: Metoprolol was found to be significantly effective in reducing and/or preventing spinal cord ischemiareperfusion injury.
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    Therapeutic Treatment with Abdominal Adipose Mesenchymal Cells Does Not Prevent Elastase-Induced Emphysema in Rats
    (2020) Gülhan, Pınar Yıldız; Ekici, Mehmet Savaş; Ekici, Aydanur; Niyaz, Mehmet; Gülhan, Muhammet; Ercin, Mustafa Emre; Aksoy, Nurkan
    OBJECTIVES: Emphysema and chronic bronchitis have different pathophysiologies but both are significant components of chronic obstructive lung disease (COPD). The levels of Matrix metalloproteinase (MMP)-9 in the bronchoalveloar lavage fluid (BALF) and in serumindicate the presence of emphysema. Intratracheal administration of elastase has been used to create a rat model of emphysema. Adiposetissue-derived mesenchymal stem cells (MSC) have been postulated to prevent or reverse emphysema, however, this has not been examined in the rat model of elastase-induced emphysema.MATERIALS AND METHODS: In this study, 31 Wistar albino rats aged 6–8 weeks and weighing 250–300 g were assessed. On day 1, theanimals were treated intratracheally with 0.5 mL saline (control group, n=10), i.e., 0.5 mL saline solution containing 0.1 IU porcine pancreatic elastase (PPE) (Elastase group, n=12) or PPE plus MSC (Elastase-MSC group, n=9) was adminstered per animal. MSCs suspendedin serum were injected via the caudal vein on day 21. At least 106 cells were injected. All animals were sacrificed on day 42 and theemphysema index (EI) was calculated, along with measuring the BALF and serum MMP-9 concentrations.RESULTS: Porcine pancreatic elastase induced a significant degree of emphysema in the PPE groups as compared to the control group,which was determined by the EI index (p=0.008). This was not reversed by MSC treatment. The EI remained significantly low in comprison with the controls (p=0.001) and measured no different from the Elastase-treated animals. There was no statistically significantdifference between the BALF and serum MMP-9 levels between the control and treatment groups.CONCLUSION: Our findings suggest that therapeutic treatment with adipose tissue-derived MSC in rats has no effect on emphysema oron MMP9 expression, which is a known marker of emphysema.