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Öğe Expression of Concern: Genetic Diversity Among Historical Olive (Olea europaea L.) Genotypes from Southern Anatolia Based on SSR Markers(Springer/Plenum Publishers, 2019) Sakar, Ebru; Ünver, Hülya; Ercişli, Sezai…Öğe Genetic Diversity Among Historical Olive (Olea europaea L.) Genotypes from Southern Anatolia Based on SSR Markers(Springer/Plenum Publishers, 2016) Sakar, Ebru; Ünver, Hülya; Ercişli, SezaiOlive (Olea europaea) is an ancient and important crop in both olive oil production and table use. It is important to identify the genetic diversity of olive genetic resources for cultivar development and evaluation of olive germplasm. In the study, 14 microsatellite markers (UDO4, UDO8, UDO9, UDO11, UDO12, UDO22, UDO24, UDO26, UDO28, DCA9, DCA11, DCA13, DCA15, and DCA18) were used to assess the genetic variation on 76 olive (Olea europaea L.) genotypes from Mardin province together with 6 well-known Turkish and 4 well-known foreign reference cultivars. All microsatellite markers showed polymorphism and the number of alleles varied between 9 and 22, with an average of 14.57. The most informative loci were DCA 11 (22 alleles) and DCA 9 (21 alleles). Dendrogram based on genetic distances was constructed for the 86 olive genotypes/cultivars, which revealed the existence of different clusters. The high genetic similarity was evident between Bakirkire2 and Zinnar5 (0.74) genotypes, while the most genetically divergent genotypes were Gurmese5 and Yedikardes, ler2 (0.19). It was concluded that there was abundant SSR polymorphism in olive germplasm in southern Anatolia in Turkey and could be important for future breeding activities.Öğe Genetic Diversity and Relationships among Local Olive (Olea europeaea L.) Genotypes from Gaziantep Province and Notable Cultivars in Turkey, Based on SSR Markers(Univ Agr Sci & Veterinary Med Cluj-Napoca, 2016) Sakar, Ebru; Ünver, Hülya; Ulaş, Mehmet; Lazovic, Biljana; Ercişli, SezaiOlive and olive oil have a prominent place in the cultures of the countries within the Mediterranean basin including Turkey. The genetic relationships among 30 olive (Olea europaea L.) genotypes sampled from Gaziantep province in Turkey were examined using 10 simple sequence repeat (SSR) markers (DCA9, DCA11, DCA15, DCA18UD04, UDO9, UD011, UDO12, UD022, UD024). Also, three well known Turkish and one foreign olive cultivar were also included within the SSR analysis. The number of alleles per locus of the SSR markers ranged from 5 (DCA15, UDO9) to 14 (DCA9) (average 7.9), for a total of 79 alleles. Similarity coefficients were calculated on the basis of 79 amplified bands. A dendrogram was created according to the 10 SSR markers by the unweighted pair-group method. The banding patterns obtained from the SSR primers allowed all of the genotypes/cultivars to be distinguished. According to the dendrogram, the 33 olive genotypes and cultivars were clustered into five main clusters. The most closely related genotypes were 'Oguzeli 3' and Tavuzeli with 0.80 similarity ratio. The most genetically divergent cultivars were 'Yavuzeli 6' and `Kilis Yaglik' (0.30), Tavuzeli 6' and 'Sauranis (0.20), Nizip 7' and Tavuzeli 4' (0.15), Islahiye 5' and Nizip Yaglik' (0.10). In conclusion, SSR analysis can be an efficient method for olive genotypes and cultivar identification and can offer valuable informative data to identify olive genotypes and cultivars grown in Turkey.Öğe MOLECULAR CHARACTERIZATION OF LOCAL OLIVE GENOTYPES FROM SOUTHERN ANATOLIA FORESTS(Publ House Bulgarian Acad Sci, 2018) Sakar, Ebru; Özkaya, Mücahit Taha; Ergül, Ali; Ünver, Hülya; Ulaş, Mehmet; Toteva, Veneta Kapchina; Ercişli, SezaiTurkey is one of the major olive (Olea europeaea L.) producers in the world and the country has a wide variety of olive germplasm including local genotypes, local cultivars, and standard cultivars as well. However, there is little information on genetic structure of local olive genotypes that are extensively grown especially in southern Anatolia region in Turkey. We examined the genetic relatedness of 58 local Turkish olive genotypes sampled from forests of Sirnak province located in southern Anatolia by using 10 SSR (simple sequence repeat) markers that were previously developed for olive and widely used in different laboratories to assess genetic diversity and relatedness in olive. In the study 4 introduced foreign cultivars along with 6 well-known standard Turkish cultivars are also included in SSR analysis to make comparison with local genotypes. Allele diversity ranged from 8 (UDO9) to 24 (DCA9), (DCA18) an average 17.6 per SSR loci presenting high polymorphism and were found to be higher than in previous studies that used the same loci for olive. No relationship was found between the genetic relatedness and the geographical distributions of these genotypes and cultivars. The data reported here may be useful to prevent confusions of Turkish olive germplasm and could be important in future breeding activities.Öğe Şırnak İli Zeytin Gen Kaynaklarının Morfolojik, Pomolojik Özellikleri İle Yağ Asidi Kompozisyonlarının Belirlenmesi(2017) Sakar, Ebru; Ünver, Hülya; Ulaş, Mehmet; Ercişli, SezaiÜlkemiz Güneydoğu Anadolu Bölgesi'nde yer alan Şırnak ilindeki zeytin gen kaynaklarının oluşturduğu populasyon içerisinden üstün nitelikli olanları belirlemek amacıyla gerçekleştirilen çalışmada, 34 genotipten sürgün, yaprak ve meyve örnekleri alınmıştır. Belirlenen genotiplerde ağaç, meyve ve yaprak özellikleri ile toplam yağ ve yağ asitleri kompozisyonları incelenmiştir. Şırnak ili genotiplerinin çoğunlukla dik ve yarı dik taç yapısında ve tiplerin çoğunluğunun eliptik uzun şekilli yaprağa sahip oldukları görülmüştür. İncelenen tiplerde meyve ağırlığı 0.70 g (Deran5)-4.20 g (Serekani) ve meyve şekli tiplerin çoğunluğunda eliptik olarak belirlenmiştir. Toplam yağ oranı % 2.0 ile % 8.8 arasında bulunmuştur. Yağ asitleri miktarları, palmitik asit %12.57 (Karkamış3)-%19.82 (Oğuzeli1), stearik asit %2.31 (Islahiye1)%4.23 (Araban2), oleik asit %58.68 (Oğuzeli3)-%72.86 (Karkamış3), linoleik asit %5.10 (Araban1)-%21.06 (Oğuzeli3) ve linolenik asit %0.73 (Oğuzeli1)-%1.71 (Nizip9, Nizip10) olarak belirlenmiştir
