Yazar "Emire, Zuhal" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    Determination of Cellulase Enzyme Produced by Bacillus cereus DU-1 Isolated from Soil, and Its Effects on Cotton Fiber
    (Inst Tecnologia Parana, 2024) Ugras, Serpil; Bicen, Hatice Elif Isik; Emire, Zuhal
    A Cellulase enzyme is the enzyme synthesized by some bacterial and fungal species and breaks down the ss-1,4 glycoside bonds of cellulose. Cellulase enzyme has a wide range of uses such as food, paper, textile industry, animal feed and detergent production. In this study, which was designed to add a new cellulase enzyme with such a wide industrial use, cellulotic bacterium isolation, identification, partial purification, characterization and application on denim fabric of the cellulase enzyme synthesized by the identified bacterium was performed. Firstly, bacterium that can produce cellulase enzyme was isolated from soil samples containing hazelnut wastes. The enzyme-producing isolate was identified as Bacillus cereus strain DU -1 by morphological, biochemical and molecular techniques. The optimum conditions for enzyme production by B. cereus DU -1 was determined as pH 7.0, at 37 degrees C and 24h. However, the activity of the enzyme (5.16 U/mL) was found to be optimum at 50 degrees C and pH 6.0. The Vmax value of the enzyme was 3.18 U/mL and the Km value was 0.0019 mM. The enzyme was partially purified by ammonium sulfate precipitation. The molecular size of the partially purified enzyme was determined as approximately 40 kDa by SDS-PAGE and Zymogram analysis. Finally, enzyme was applied on the denim fabric and when the fabrics were viewed under the electron microscope, it was seen that they were suitable for textile use.
  • Küçük Resim Yok
    Öğe
    Protective Effects of Origanum onites and Its Components on Lead-Nitrate Induced Genotoxicity in Root Cells of Allium cepa L.
    (Acg Publications, 2024) Ugras, Serpil; Rasgele, Pinar Goc; Temizce, Semih; Emire, Zuhal; Dirmenci, Tuncay
    This study investigates the protective effects of components (Thymol; Thy, Carvacrol; Car, Linalool; Lin, and alpha-Pinene; AP) and essential oil of Origanum onites L. (O. onites-EO), against lead nitrate -induced cytotoxicity and genotoxicity in Allium cepa L. (A. cepa) root tip cells. These components obtained from O. onites were characterized by gas chromatography (GC). A. cepa bulbs were exposed to 6.25-12.5 mg/L concentrations of the O. onites-EO/components of O. onites-EO for analyses of induction of cytogenetic damage. Then, these bulbs were exposed to 10 mg/L concentrations of lead nitrate for analyses of the protective effects of O. onites and its components. Mitotic abnormalities were evaluated for genotoxicity, and mitotic index (MI) for cytotoxicity. As a result of this study, lead nitrate increased the total chromosomal abnormality amount in A. cepa, indicating genotoxicity. MI was decreased with lead nitrate. However, this effect was significantly improved by components of O. onites-EO. This effect was shown with the decrease in the number of chromosomal abnormalities and increase in MI rates in lead nitrate -induced root cells after exposure to the components of O. onites-EO. The protective effect of O. onites-EO components against the damage caused by lead nitrate in cells can be listed as alpha- Pinene > Thymol > Carvacrol > Linalool. Among all essential oil components tested, alpha-Pinene was determined to have the strongest protective effect. Furthermore, the protective effect of the essential oil, which contains all the components, could not be determined. It has been observed that the components of essential oil have different effects, and it can be said that these components suppress the effects of each other in the mixture where they are found together. In conclusion, this study shows that the components of O. onites-EO have a protective effect on lead nitrate -induced A. cepa root cells.