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    Molecularly Imprinted Polymer Based Biosensor For Choline
    (2020) Polat, Tuğçe; Bakay, Melahat Sevgül; Denizli, Adil; Utku, Feride Şermin
    Biosensors are systems that can perform quantitative and/or qualitative analysis of substances in liquid or gasenvironment through their biological recognition sites and transform the acquired data into detectable signals.Biosensors are able to detect physical changes (i.e. as density, mass concentration, etc.) by means of recognitionsites and correlate them with electrical or optical quantities (i.e. current, voltage and impedance). In this study,three molecularly imprinted pencil graphite electrodes with differing numbers of choline recognition sites, at E-1M, E-3 M and E-5 M concentration, were used as electrochemical biosensors. An increase in choline receptorconcentration on the electrode surface was expected to correlate with an increase in PGE surface bound cholineand thus lead to electrical changes. The study was conducted in a three-electrode cell with Ag/AgCl as thereference electrode, platinum wire as the counter electrode and PGE as the working electrode. Cyclicvoltammetry and electrochemical impedance measurements were conducted in 10 mM phosphate buffer solutioncontaining 5mM K3[FeCN6]-3/-4redox pair. As expected, as increasing amount of choline was bound to thecomplementary recognition sites on choline imprinted electrodes, a correlating change in current, voltage andimpedance was observed. The dynamic detection range for choline expanded as the choline concentrationimprinted on the electrodes increased. Using the E-1 M PGE electrode, 72 pM limit of detection, up to 7.2 nMlimit of linearity was attained.