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    Comparison of HPLC and turbidimetric immuno-inhibition method for determination of HbA1c
    (Düzce Üniversitesi, 2007) Coskun, Abdurrahman; Yavuz, Özlem; Memısogulları, Ramazan; Kurt, Hatice
    Purpose: Measurement of HbA1c is widely accepted as retrospective index of glycemic control in Diabetes Mellitus (DM) patients. Currently several different methods are used in clinical laboratories for measurement of HbA1c. However most of these methods are complicated and expensive. In the present study we aimed to make a comparison between HPLC and turbidimetric immuno-inhibition methods for determination HbA1c to decide the more suitable one for clinical laboratories. Methods: One hundred and seventy eight patients with DM were included into the study. Blood levels of HbA1cwere determined by both HPLC and turbidimetric immuno-inhibition methods. Data obtained from two methods were compared with various statistical techniques. Results: There was no significant diffe rence between HbA1c results measured with HPLC (%6.27±2.0) and turbidimetric immuno-inhibition (%6.31±2.2) methods (p gt;0.05). We also observed a strong positive corelation between both methods (r = 0.934, p lt; 0.001). Conclusion: In clinical laboratory practice HPLC method is complicated, time consuming and expensive. However turbidimetric immuno-inhibition method is simple, chip and could be apply to most autoanalyzers which is common in clinical laboratories. We concluded that turbidimetric immuno-inhibition method should be preferred to HPLC method for measurement of HbA1c for monitoring glycemic control in Diabetes Mellitus patients