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Öğe Evaluation of the Protective Effect of the Cup Therapy on the Epileptic Seizure in Rats(Duzce Univ, Fac Medicine, 2021) Cetinkaya, Ayhan; Karamaden, Esra Fidan; Goksu, Selim; Bozat, Bihter Gokce; Demir, SerifObjective: Cup therapy has an important place in traditional and complementary medicine applications. The purpose of our study, this is the first time to investigate the protective effect of cup therapy in rats on experimentally generated epileptic seizures in new rat modeling created by different anatomic regions. Methods: A total of 42 Wistar albino rats were randomly divided into 6 groups (n:7). The new dry cup was applied to the G1 group, and the new wet cup model was applied to the G2. In the G3 group, an epilepsy model (PTZ, 35 mg / kg) was created and diazepam (2.5 mg / kg) was given to G4. Dry cup and wet cup models were applied to the G5 and G6 groups, respectively. 24 hours after the cupping therapies, the rats were injected with PTZ and the epilepsy behavior scores of the rats in all groups were recorded for 20-30 minutes. Results: In the 'Open Area' and 'Elevated Plus Maze' tests, there was no behavioral difference between the cup therapy group and the control group (p > 0.05). Given all the parameters, the G4 group significantly reduces the seizure compared to other groups (p < 0.05). There is a significant difference in G2, G5 and G6 groups compared to G3 in the phases parameter (p < 0.05). Conclusions: In this study, the new wet cup therapy (G2), which was applied for the first time, had a protective effect on seizures. G2, G5 and G6 groups are observed to suppress seizures compared to G3. Our findings are expected to contribute greatly to animal model analysis in the future.Öğe Investigation of penicillin-induced epilepsy model in female rats with different estrous cycles by ECoG method(Wolters Kluwer Medknow Publications, 2023) Cetinkaya, Ayhan; Kara, Sinem; Bozat, Bihter; Yabalak, AhmetAim: Epilepsy is a chronic neurological disease characterized by recurrent seizures resulting from an imbalance in neuronal excitation. It is known that there is a tendency for seizures to cluster in both men and women at certain periods. This study aims to compare electrocorticogram (ECoG) recordings in an experimentally induced epilepsy model using penicillin, conducted at different estrous cycles of female rats. Subjects and Methods: Prior to commencing the epilepsy experiments, vaginal smears were collected from the animals every morning for 1 week, at the same time each day, for cytological examination. After confirming that the estrous cycles (proestrus, estrous stage, metestrus, and diestrus) of the animals were normal and healthy, the experiment was initiated. Additionally, H and E staining was performed on the ovaries of the rats sacrificed at the end of the experiment to validate the estrous cycle phases. A total of 32 adult female Wistar rats were divided into four groups (proestrus, estrus, metestrus, and diestrus), each consisting of eight rats. The penicillin-induced epilepsy model involved anesthetizing the rats with 1.25 mg/kg urethane intraperitoneally, followed by the injection of penicillin G (500 IU, 2.5 mu L, i.c.) into the cortex region through a skull opening. ECoG recordings were conducted using PowerLab analysis for 180 min. Statistical analysis of the results was performed using the SPSS program version 22 (IBM Corp., Armonk, NY, USA). One-way analysis of variance (ANOVA) and independent samples t-test were employed for groups with normally distributed data, while the Kruskal-Wallis test and Mann-Whitney U-test were used for groups with nonnormally distributed data. Statistical significance was set at P < 0.05. Results: Histological evaluation confirmed that vaginal smears and ovarian histopathology were consistent with each other, validating the data pertaining to the groups. In the ECoG recording results, the spike-wave frequency between 6 and 10 min was higher in the metestrus group compared to the proestrus (P = 0.004), estrus (P = 0.046), and diestrus (P = 0.025) groups. No significant differences were observed among the groups in terms of spike-wave amplitude and latency. Conclusion: Our study using a penicillin-induced epilepsy model in female Wistar rats demonstrated that the spike-wave frequency was highest during the metestrus phase. This finding provides valuable insights for future studies aiming to understand and mitigate the sensitivity observed during the metestrus phase, which is characterized by rapid progesterone withdrawal.Öğe Protective Effect of Cup Therapy Model in Rats on Epileptic Seizures by Determination of Different Anatomic Region(Wiley, 2019) Cetinkaya, Ayhan; Fidan, Esra; Goksu, Selim; Bozat, Bihter Gokce; Demir, Serif[Abstract Not Available]
