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    Effect of magnetic field on in vitro seedling growth and shoot regeneration from cotyledon node explants of Lathyrus chrysanthus boiss
    (Wiley, 2018) Bahadır, Anzel; Beyaz, Ramazan; Yıldız, Mustafa
    The stimulatory effects on germination of seeds and growth of plants of static magnetic field (MF) pre-treatments depending on MF intensity, exposure time periods, signal form, flux density, and source frequencies on plants are reported. Seed germination frequency is low due to dormancy in Lathyrus chrysanthus Boiss. from Fabaceae family, consisting of 187 taxa. Tissue culture protocol for this plant has already been optimized. This plant is also used as a model for developing alternative methods to overcome dormancy. This study was conducted to determine the effects of MF on in vitro seed germination, seedling growth, and shoot regeneration capacity of cotyledon node explants in Lathyrus chrysanthus Boiss. to obtain healthy seedlings in large quantities. The seeds of an ecotype (Diyarbakir) were subjected to 125mT MF strength for different exposure time periods (0-untreated, 24, 48, and 72h). Sterilized seeds were germinated on growth basal medium in Magenta vessels. Seed germination and seedling growth percentages were recorded after 7 and 14 days of culture initiation, whereas seedling and root lengths were noted 28 days after culture initiation. At the end of the culture, shoot regeneration percentage, shoot number per explant, highest shoot height per explant, and total shoot number per petri dish were recorded. According to the results, it could be concluded that MF treatment could clearly be used to improve germination by breaking dormancy not only in Lathyrus chrysanthus Boiss. but also other plant species. Bioelectromagnetics. 39:547-555, 2018.(c) 2018 Wiley Periodicals, Inc.
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    The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.)
    (Czech Academy Agricultural Sciences, 2019) Aycan, Murat; Beyaz, Ramazan; Bahadır, Anzel; Yıldız, Mustafa
    This study was conducted to determine the effects of magnetic field (MF) strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.). Seeds of flax cv. Madaras were exposed to different MF strengths (0 - control, 75, 150, and 300 millitesla (mT)) for 24 h by using an electromagnetic generator system fabricated in laboratory conditions. After sterilization, seeds were germinated on MS (Murashige and Skoog) medium in Magenta vessels. Hypocotyl explants excised from 7-days-old seedlings were used for regeneration. GV2260 strain of Agrobacterium tumefaciens was used in transformation studies. Inoculated hypocotyls were cultured on MS medium containing 1 mg/l BAP (6-benzylaminopurine) and 0.02 mg/l NAA (naphthaleneacetic acid) for 2 days by co-cultivation. Then, they were transferred to MS medium containing the same growth regulators, 100 mg/l kanamycin and 500 mg/l Duocid for selection. The presence of the nptII gene was verified by PCR (polymerase chain reaction) analysis in putative transgenic plants. The highest results with respect to shoot regeneration and transformation frequency were obtained from treatments of 75 mT MF strength.
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    Magnetic field effect on breaking tuber dormancy, early sprouting, seedling growth, and tuber formation in potato (Solanum tuberosum L.)
    (Science Society Thailand, 2020) Bahadir, Anzel; Sahin, Nilufer Kocak; Beyaz, Ramazan; Yildiz, Mustafa
    Magnetic field (MF) treatment improves the germination of seeds and enhances the performance of various crops. In this study, the effects of different MF strengths (0-control, 75, 150, and 300 mT) and exposure time periods (0-control, 24, 48, and 72 h) on sprouting of dormant seed potato tubers, vegetative growth (emergence time and plant height), tuber formation (tuber number per plant and mean tuber weight), and total chlorophyll content in 2 potato (Solanum tuberosum L.) cultivars ('Necta' and 'Banba') were investigated in all parameters examined in both cultivars, the worst results were recorded in control treatment where no MF strength was used. Emergence times of sprouts were reduced significantly when seed potato tubers were exposed to 150 mT MF strength for 72 h in both cultivars. The fastest emergence times were recorded as 14.0 days in cv. 'Nectar' and 17.0 days in cv. 'Banba' when seed tubers were exposed to 150 mT MF strength for 72 h. In control treatment, emergence time of sprouts was noted as 31.8 days in cv. 'Nectar' and 39.5 days in cv. 'Banba'. The best results in other parameters (plant height, total chlorophyll content, tuber number per plant and mean tuber weight) were again obtained from seed tubers treated with 150 mT MF strength for 72 h whereas the worst results were noted in control treatment in both cultivars. Thus, MF pre-treatment can compensate for the negative effects of dormancy in seed potato tubers.