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    Automated Purification of DNA from EDTA-Preserved Whole-Blood Samples: Comparison with Manual DNA Extraction
    (Düzce Üniversitesi, 2008) Yıldırım, Abdulkadir; Turhan, Hamdullah; Yanmaz, Vefa; Bakan, Ebubekir
    In recent years, the use of PCR-based molecular techniques in the clinical laboratories has increased. For this purpose, automated DNA isolation instruments using magnetic bead technology have been developed. However, if magnetic particles are not removed sufficiently from DNA solution, amplification reactions will be inhibited by the magnetic beads, which decrease PCR sensitivity or lead to false negative PCR results. The aim of this study was to evaluate PCR-hybridization performance,productivity and purity of genomic DNA extracted by the automated system based on magnetic separation technology, and to compare these results with those obtained with manual DNA isolation method. The yield of genomic DNA extracted by the automated system (QuadroProbe QP10) from human EDTA-preserved whole blood samples was 28 to 74 ng/ l and the A260/A280 ratio was 2.5 ± 0.73. The results obtained from PCR-hybridization showed that the final solutions of DNA isolated by magnetic bead technology did not contain any inhibitory material for the PCR-hybridization reactions. These results show that QuadroProbe QP10 can be used for purification of good quality DNA from whole blood samples in PCR laboratories
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    Effect of Metformin or Gliclazide on Lipid Peroxidation and Antioxidant Levels in Patients with Diabetes Mellitus
    (Tubitak Scientific & Technical Research Council Turkey, 2008) Memişoğulları, Ramazan; Türkeli, Mehmet; Bakan, Ebubekir; Akçay, Fatih
    Aim: The association between oxidative stress and diabetic complications is well known. The main goal of antidiabetic therapy is to prevent the complications of diabetes. Gliclazide (sulfonylurea) and metformin (biguanide) are two oral antidiabetic drugs that have been proven to prevent diabetic complications. In this study, we aimed to investigate the antioxidant effects of gliclazide or metformin. Materials and Methods: In this study. we evaluated 46 patients with type 2 diabetes mellitus. The patients were divided into three groups according to their treatment modality of gliclazide, metformin, or diet. Erythrocyte glutathione peroxidase (Gpx). glutathione S-transferase (GST), and catalase (CAT) activities and malondialdehyde (MDA) and glutathione (GSH) levels of the patients were measured and compared. Results: The erythrocyte Gpx, GST, and CAT activities were significantly higher, whereas MDA levels were significantly lower in both gliclazide and metformin groups when compared with the diet group. There was no statistically significant difference between gliclazide and metformin groups in terms of activities of antioxidant enzymes and levels of MDA. In addition, GSH levels were not different among the groups. Conclusions: The data obtained in this study showed that gliclazide or metformin administration may decrease oxidative stress. and both drugs had similar effects.