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    Anti-adherent activity of nano-coatings deposited by thermionic vacuum arc plasma on C. albicans biofilm formation
    (Sage Publications Ltd, 2023) Ersoz, Mirac Berke Topcu; Mumcu, Emre; Avukat, Esra Nur; Akay, Canan; Pat, Suat; Erdonmez, Demet
    Background: The purpose of this study was to analyze the anti-adherent activity of nano-coatings deposited by Thermionic Vacuum Arc plasma on C. albicans ATCC 10231 biofilm. Materials and methods: A total of 80 disc-shaped (2 x 10 mm) polymethymethacrylate samples were prepared and divided into four groups with 10 samples in each group (Control, ZnO, SnO2, Ag) (n = 10). Using thermionic vacuum arc plasma, they were coated with ZnO, SnO 2, and Ag. 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Crystal Viole (CV) assays were conducted for biofilm quantification. Scanning electron microscopy (SEM) was used to observe biofilm images of C. albicans biofilm. Results: MTT and CV mean values differ statistically significantly between all groups (p <= 0.05). The SnO2 group had the lowest mean value, whereas the control group received the highest value. Conclusion: SnO2 coating shown greater anti-adherent activity than either metal oxides. C. albicans biofilm formation on denture base surfaces is reduced following Thermionic Vacuum Arc plasma coating with SnO2.
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    Could Helium Plasma Treatment be a Novel Approach to Prevent the Biofilm Formation of Candida albicans?
    (Springer, 2023) Avukat, Esra Nur; Akay, Canan; Ersoz, Mirac Berke Topcu; Mumcu, Emre; Pat, Suat; Erdonmez, Demet
    There is no definitive method to prevent Candida albicans (C. albicans) biofilm formation on polymethyl methacrylate (PMMA) surfaces. The objective of this study was to evaluate the effect of Helium plasma treatment (before the application of removable dentures to the patient) to prevent or reduce C. albicans ATCC 10,231 the anti-adherent activity, viability, and biofilm formation on PMMA surfaces. One hundred disc-shaped PMMA samples (2 mm x 10 mm) were prepared. The samples were randomly divided into 5 surface groups and treated with different concentrations of Helium plasma: G I: Control group (untreated), G II: 80% Helium plasma-treated group, G III: 85% Helium plasma-treated group, G IV: 90% Helium plasma-treated group, G V: 100% Helium plasma-treated group. C. albicans viability and biofilm formations were evaluated using 2 methods: MTT (3-(4,5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide) assays and Crystal Violet (CV) staining. The surface morphology and C. albicans biofilm images were observed with scanning electron microscopy. The Helium plasma-treated PMMA groups (G II, G III, G IV, G V) observed a significant reduction in C. albicans cell viability and biofilm formation compared with the control group. Treating PMMA surfaces with different concentrations of Helium plasma prevents C. albicans viability and biofilm formation. This study suggests that Helium plasma treatment might be an effective strategy in modifying PMMA surfaces to prevent denture stomatitis formation.
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    Evaluation of the effect of new generation denture base materials aged in artificial saliva at different pH levels on surface roughness and Candida albicans adhesion
    (Bmc, 2025) Arslan, Ezgi; Akay, Canan; Erdonmez, Demet; Avukat, Esra Nur
    Purpose The aim of this in vitro study was to evaluate the surface roughness and Candida albicans (C. albicans) biofilm formation of conventional heat-cured and different CAD/CAM milling denture base materials, after immersion in different pH levels of artificial saliva. Materials and methods Disc-shaped samples (10 x 2 mm) (n = 10) were prepared from two brands of CAD/CAM milled and conventional heat-cured PMMA denture base materials. The samples were immersed at 37 degrees C for 21 days in different pH levels of artificial saliva. The surface roughness (Ra) of each sample was measured using a contact profilometer before and after aging. C. albicans adhesion of the samples was determined using the Crystal Violet (CV) method. Results A significant increase in roughness was observed across all material groups maintained at pH 3 (P < 0.001). At pH 7, the roughness for heat-cured PMMA and G-PMMA groups increased significantly. Although pH 14 increased roughness in all groups, this was not significant. Upon comparison of roughness values post-aging, no significant differences were noted between the groups at pH 3 and pH 7; however, the G-PMMA group exhibited significantly lower surface roughness at pH 14 (P < 0.05). Among all material groups, the highest adhesion of C. albicans was observed in the heat-cured PMMA group. (P < 0.05). Conclusions Aging at different pH values caused surface changes in denture base materials and differences in C. albicans adhesion. Although there is an increase in surface roughness in all groups, these values are lower than the plaque accumulation threshold. The highest C. albicans adhesion was observed in heat-cured PMMA samples. Considering both roughness values and Candida adhesions, it is concluded that the new generation denture base materials are suitable for clinical use and should be widely used.