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  • Küçük Resim
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    Determination of taxanes by validated LC-MS/MS method in hazelnut collected from different regions and altitudes in Turkey
    (Acg Publications, 2018) Oğuzkan, Sibel Bayıl; Karagül, Bora; Aksoy, Elif Sine; Uzun, Ayşe; Can, Merve; Yılmaz, Hasibe; Gören, Ahmet C.
    In this study, mass fractions of paclitaxel, cephalomannine, 10-DAB III and baccatin III in hazelnut were measured with new and validated LC-MS/MS method. Green outer nut husk and nutshell of Turkish hazelnut (Corylus colurna L.) were collected from Duzce, Trabzon, Adapazari and Samsun at several altitudes during August and September in 2014. The target analytes were prepared using solid-liquid extraction and analysed by LC-MS/MS. High amount of baccatin III, used for synthesizing semi-synthetic paclitaxel, was found in the nutshells collected at 250-500 m altitude in Cumayeri district of Duzce. In the husk samples from Vakfikebir district of Trabzon, the highest amount of baccatin III was obtained. The mass fractions of baccatin III in nutshell and husks were between 164.38-1020.85 mu gkg-1 and 166.12-923.64 mu gkg-1, respectively.
  • Küçük Resim
    Öğe
    Effects of Some Adsorbents on the Pre-purification of Taxol (Anticancer Drug) from Hazelnut Nutshells
    (Asian Network Scientific Information-Ansinet, 2018) Oğuzkan, Sibel Bayıl; Karadeniz, Şeref; Karagül, Bora; Uzun, Ayşe; Aksoy, Elif Sine; Güler, Özen Özensoy; Uğraş, Halil İbrahim
    Background and Objective: The most important pharmaceutically active substance in plant-derived chemotherapy drugs is paclitaxel. Taxane compounds such as paclitaxel, cephalomannine, baccatin III and 10-Deacetylbaccatin III are found in hazelnut shells of Turkey's Black Sea region. This study aimed to determine the most effective adsorbent on the purity and efficiency of these taxane compounds from nutshells. Materials and Methods: The adsorbent effect on the purification of taxane compounds from hazelnut shells was studied using different adsorbent materials. For this purpose, extracts were obtained from samples of hazelnut shells collected, dried and ground into solvents. The resulting extract was concentrated into a stock solution. Samples taken from the stock solution were separately treated with seven different adsorbent materials and then filtered and analyzed with HPLC (High Performance Liquid Chromatography). Results: The HPLC analysis of taxane compounds determined on taxane, both the purification and whether or not the substance was lost, by measuring the efficiency at the same time. Graphene-oxide was found to be the adsorbent with the highest positive effect on the purification of paclitaxel in hazelnut nutshells in all cases. Graphene-oxide also had the least loss in terms of paclitaxel yield efficiency in hazelnut nutshells. Conclusion: The effects of adsorbent treatment increased both the purity and efficiency of taxane compounds and was, thus, a useful pre-purification method for shells obtained from Turkish hazelnuts. This shell waste material may prove to be a good alternative source of taxol as an anticancer drug.
  • Küçük Resim
    Öğe
    Fındık kabuğundan paklitaksel saflaştırılması
    (Düzce Üniversitesi, 2017) Aksoy, Elif Sine; Uğraş, Halil İbrahim
    Kalp hastalıklarından sonra dünya genelinde yaygın olan ve ölümle sonuçlanan hastalıklardan biri olan kanser günümüz sağlık problemlerinin başında geldiği, yapılan çalışmalarca kanıtlanmıştır. Birçok tedavi şekli olan kanser hastalığında en çok kullanılan yöntem kemoterapidir ve bu yöntem için çok sayıda ilaç geliştirilmiştir. Çalışmamızda bunlardan biri olan paklitaksel üzerine izlolasyon çalışmaları amaçlanmıştır. Paklitakselin fındık kabuğundan izolasyonu kromatografik saflaştırma çalışmaları; farklı çözücüler ve farklı kolon dolgu maddeleri kullanarak ters faz ve normal faz kolon uygulamaları yapılarak optimizasyon denemeleri gerçekleştirilmiştir. Paklitakselin fındık kabuğunda varlığı yapılan analizlerle kanıtlanmıştır. Denemelerde olumlu, olumsuz ve geliştirilebilecek durumlar belirlenmiştir. Fındık kabuğunda; önemli bir anti kanser ilacı olan paklitakselin varlığı bize ülkemizde oldukça fazla üretilebilen ve sürekliliği olan aynı zamanda çoğunlukla atık olarak kullanılan kabuğun çok daha değerli hale getirilebileceğini göstermiştir.