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    Chemical Composition of Lamium purpureum L. and Determination of Anticancer Activity of Its Essential Oil on Melanoma
    (2019) Akkoyunlu, Aysegul; Dülger, Görkem
    The current study aimed to evaluate Lamium purpureum L. in terms of its chemical composition and thepotential of its essential oil for anticancer activity. The profiling analysis of its chemical composition was carriedout via GC-MS. For this purpose, the ethanol extract was subjected to GC-MS analysis to determine chemicalcompounds. To obtain essential oil, steam distillation of the whole plant (aerial, leaf, stem) was carried out in aClevenger apparatus. The anticancer activitiy was determined via MTT assay. We observed that the maximumcell death was 14% at 50 µg/mL concentration. As a result of GC-MS analysis, palmitic acid, 7-tetradecenal,octadecadienoic acid, acetic acid, ethyl 9,12,15-octadecatrienoate, hexatriacontane, stearate and benzyl benzoatewere identified as major components. We determined that the essential oil of L. purpureum L. had essentialcompounds which showed cytotoxic activities.
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    Exploring the antibiofilm effects on Escherichia coli biofilm associated with colon cancer and anticancer activities on HCT116 cell line of bee products
    (Taylor & Francis Ltd, 2024) Akkoyunlu, Aysegul; Dülger, Görkem
    The association between dysbiotic microbiota biofilm and colon cancer has recently begun to attract attention. In the study, the apitherapeutic effects of bee products (honey, bee venom, royal jelly, pollen, perga and propolis) obtained from the endemic Y & imath;& gbreve;& imath;lca ecotype of Apis mellifera anatoliaca were investigated. Antibiofilm activity were performed by microplate assay using crystal violet staining to measure adherent biofilm biomass of Escherichia coli capable of forming biofilms. Bee venom showed the highest inhibition effect (73.98%) at 50% concentration. Honey, perga and royal jelly reduced biofilm formation by >50% at all concentrations. The antiproliferation effect on the HCT116 colon cancer cell line was investigated with the water-soluble tetrazolium salt-1 assay. After 48 h of honey application at 50% concentration, cell proliferation decreased by 86.51%. The high cytotoxic effects of royal jelly and bee venom are also remarkable. Additionally, apoptotic pathway analysis was performed by ELISA using caspase 3, 8 and 9 enzyme-linked immunosorbent assay kits. All bee products induced a higher expression of caspase 9 compared with caspase 8. Natural products that upregulate caspase proteins are promising therapeutic targets for proliferative diseases.