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    Antibiofilm, antiquorum sensing and antioxidant activities of Calendula officinalis L., Hypericum perforatum L. and Trachystemon orientalis (L.) G. Don essential oils
    (Natl Inst Science Communication-Niscair, 2024) Gedik, B.; Akkoyunlu, A.; Dulger, G.
    Bacteria can coordinate biofilm formation through antiquorum sensing and create a more effective defense mechanism. Therefore, strategies that inhibit biofilm formation and target the antiquorum sensing mechanism are needed to combat antibiotic resistance. This study was conducted to determine the antibiofilm and antiquorum sensing (anti-QS) effect of essential oils (EOs) obtained from Calendula officinalis L., Hypericum perforatum L. and Trachystemon orientalis (L.) G. Don. and also to calculate oxidative stress indices (OSI) by analysing oxidant and antioxidant capacity. Antibiofilm capacities of EOs against Escherichia coli were investigated by microplate method. Anti-QS activities were evaluated with the agar well diffusion test. The oxidant capacities of plant EOs, whose antioxidant value was determined with the Total Antioxidant Status kit (TAS), were also examined with the Total Oxidant Status kit (TOS), and the OSI were calculated from the ratio of the two. EOs of all three plants have strong antibiofilm and anti-QS effects. T. orientalis, which has 19 mm+ anti-QS effect, shows 93.11% biofilm inhibition effect and draws attention together with the other two plants used pharmacologically. EOs with low OSI levels are expected to have high phytotherapeutic efficacy. As expected, the EOs used are considered important components for their capacity to inhibit biofilm formation and the QS mechanism. This study constitutes the first step in using EOs as alternative agents for strategies that prevent the anti-QS mechanism and biofilm formation.
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    Chemical Composition and In Vitro Antimicrobial, Antioxidant, and Antiproliferative Studies of the Lamium galeobdolon L. (L.) Essential Oil
    (Maik Nauka/Interperiodica/Springer, 2022) Akkoyunlu, A.; Dülger, Görkem
    The current study aimed to evaluate the chemical composition and bioactive properties of Lamium galeobdolon (L.) L. After obtaining an ethanol extract, analysis of its chemical composition was carried out via gas chromatography-mass spectroscopy (GC-MS), which identified 2(3H)-benzoxazolone, palmitic acid, 3,7,11,15-tetramethyl-2-hexadecen-1-ol, eicosane, 9,12-octadecadienoic acid, and tetracosane as major components. In addition, the antioxidant and oxidant capacities of the essential oil were determined using the total antioxidant status (TAS) and the total oxidant status (TOS) kits, and the oxidative stress index (OSI) value was calculated from the ratio of the two. The OSI value of L. galeobdolon (L.) L. was found to be 0.987 arbitrary units (AU). Essential oil was obtained from the plant and the antimicrobial and antiproliferative effects of this oil were investigated. The antiproliferative activity was determined via MTT assay on the B16F10 cell line. The maximum cell death was observed as 88.9% at a concentration of 50 mu g/mL. The antimicrobial activity was determined via the disc diffusion method. Accordingly, no antibacterial activity was found; however, the antifungal effect of the plant was significant.