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Öğe The Effect Of L-Carnitine And Alpha Lipoic Acid Administration With Exercise In Old Rats On Energy Metabolism Related To Oxidative Stress Parameters(Marmara Univ, Inst Health Sciences, 2024) Kalkan, Kardelen Kocaman; Sirin, Neslihan; Tepe, Atakan; Gok, Ali; Altas, Tolga; Agan, Kagan; Gulhan, Pinar YildizObjective: This study aims to contribute novel insights by investigating the potential positive effects of a combined dietary supplement and exercise program on mitochondrial oxidative stress and energy metabolism in aging. Focusing on the protective impact of Alpha Lipoic Acid (ALA), a potent antioxidant, against exercise-induced mitochondrial oxidative stress in rats, we also assess how L-Carnitine administration affects exercise ability by analyzing resistin and HbA1c levels, indicators linked to insulin resistance and cellular sensitivity. Method: In this 10-day study, 42 old male Sprague Dawley rats (weighing 400 +/- 10 g, aged 15-17 weeks) were divided into six groups (n=7): Control, Exercise, L-Carnitine, Alpha Lipoic Acid (ALA), L-Carnitine+Exercise, ALA+Exercise. Relevant groups received daily oral gavage doses of L - Carnitine (50 mg/ml) and ALA (18 mg/ml). Exercise groups underwent treadmill sessions. On day 10, blood samples were quantitatively analyzed for HbA1c and Resistin levels using a Cusabio ELISA assay kit (China). Results: ALA supplementation synergistically reduced resistin and HbA1c levels, individually and combined with exercise. Conversely, L-Carnitine supplement, alone or with exercise, increased resistin levels but it caused a decrease in HbA1c levels. Conclusion: The data indicated a minor, insignificant decrease in resistin levels for the exercise and ALA groups, with a statistically significant difference in HbA1c levels among all groups. Exercise alone positively impacted both HbA1c and resistin levels, suggesting a potential counteraction of age-related oxidative stress and a positive influence on energy metabolism through an appropriate diet and exercise program. Further studies are required to explore specific metabolic pathways and relationships identified in our findings.Öğe Investigation of Total Phenolic Content of Tilia dasystyla and Polygonatum orientale Desf Extracts and Their Cytotoxic Effect on the Osteogenic Sarcoma (Saos-2) Cancer Cell Line(Kowsar Publ, 2020) Zarringhalami, Roshanak; Hanachi, Parichehr; Kaya, Ertugrul; Agan, Aydan Fulden; Agan, Kagan; Donmez, MertBackground: Osteosarcoma; is one of the most common malignant tumors. Nowadays, because of the many side effects of cancer drugs, the usage of herbal medicine which can inhibit or eliminate cancer cells by their antioxidant compounds is increased. Objectives: In the present study anticancer effect of Tilia dasystyla and Polygonatum orientale Desf different extracts on osteogenic sarcoma (Saos-2) cancer cell line was investigated and their polyphenolic compounds were identified by liquid chromatography-mass spectrometry (LC-MS) analysis. The cytotoxic effect of these extracts on Saos-2 cell line and identification of their phenolic compounds have not been reported so far. Methods: Cancer cell lines were provided from Department of Biological Sciences, Bursa University, Turkey. Different concentrations of the methanol, ethanol, and diluted water extracts (0.5 - 5 mg/mL) were tested on Saos-2 cell line. After 24, 48, and 72 hours, the cell viability was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. For the investigation of total phenolic compounds of T. dasystyla and P. orientale Desf extracts LC-MS method was applied. Results: According to the results diluted water extracts on the Saos-2 cancer cell line showed more cytotoxic effect than other solvents. The lowest IC50 value was 0.58 +/- 0.01 mg/mL within 72 hours belonged to T. dasystyla water extract. Conclusions: Tilia dasystyla and Polygonatum orientale Desf extracts contain some polyphenolic compounds which showed cytotoxic effect on Saos-2 cancer cell line. The full potential of these herbal extracts is yet to be realized by further studies on animal models and subsequent trials.