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Öğe Anticancer Effect of Food Supplements on Saos-2 Osteosarcoma Cell(Düzce Üniversitesi, 2020) Ağan, Kağan; Zarringhalami, Roshanak; Ağan, Aydan Fülden; Yaşar, MustafaObjective: Nowadays medicinal plants have been considered as complementary medicine in cancer treatment by many researchers. Osteosarcoma, one of the most common malignant tumors, which is more common at a young age; It is one of the most common types of cancer that occur in mesenchymal bone-forming cells and affect the musculoskeletal system. Today instead of cancer drugs due to their numerous side effects that can suppress or prevent cancer treatment, food supplement products that contain plant extracts may be preferred. In this study, the anticancer effects of five different food supplements were investigated on osteosarcoma Saos-2 cancer cell line. Material-Method: Application doses were as 1000, 2000, 3000, 4000, 5000, 6000 µg/ml and IC50 values were calculated by creating two working groups of 24 and 48 hours. Results: Extracts of five different herbal product showed cytotoxic effect on Saos-2 cancer cell line due to their mixture of medicinal plants, however REGULIN® showed the most cytotoxic effect among them.Conclusion: It was determined that REGULIN® used in the remember regeneration therapy method (RTM), had the most anticancer effect in each of the 24 and 48 hour application groups. Cytotoxic effect of these food products on other cancer cell lines and their use in the pharmaceutical and health industries is recommended in future studies.Öğe Antiproliferative Effect of IST-GLIO® Supplement Food Product on C6 Glioma Cells(Düzce Üniversitesi, 2020) Yaşar, Mustafa; Saraç, Güliz; Ağyar Yoldaş, Pınar; Ağan, Aydan Fülden; Kaya, ErtuğrulObjective: Cancer is one of the leading causes of death worldwide and is characterized by the proliferation of abnormal cells. In recent years, as in the treatment of many diseases, there is also a return to nature in the treatment of cancer. In developing societies, the concepts such as prophylaxis of disease prevention rise to prominence in order to reduce the material and moral losses caused by disease treatment. Consequently, the use of supplementary food products occupies more and more space in daily life. In this study, it was aimed to investigate the antiproliferative properties of IST-GLIO® food supplement, which is one of these products and used in the Remember Regeneration Therapy Method (RTM), on C6 (rat glioma) cells.Material-Method: WST-1 cell proliferation test protocol was applied to examine the antiproliferative effect of IST-GLIO® and the results were evaluated according to ELISA microplate reading data. The product was tested at concentrations ranging from 1 ng/mL to 100 ng/mL. Results: When the results were evaluated, it was appeared to be effective in the concentration range of 6-20 ng/mL on the C6 glioma cell culture; it was found that the efficacy didn’t occur through general toxicity, specifically reducing the reproduction of the cancer cells in question, since food supplement did not inhibit cell division further at higher doses.Conclusion: As a result, non-specific toxicity is observed in many cultures that are similarly investigated in cell cultures, depending on concentration as high doses are obtained. The IST-GLIO® product does not show this feature is considered as promising.Öğe DVD-REG® TAKVİYE EDİCİ GIDA ÜRÜNÜNÜN AKUT SUB-AKUT TOKSİSİTESİNİN SIÇANLAR ÜZERİNDE ARAŞTIRILMASI(Sağlık Bilimleri Üniversitesi, 2020) Yaşar, Mustafa; Saraç, Güliz; Sipahi, Nisa; Yoldaş, Pınar Ağyar; Ağan, Kağan; Ağan, Aydan Fülden; Şirin, NeslihanGeleneksel ve tamamlayıcı tıp alanında tıbbi olarak faydalı olduğu bilinen bitkilerin farklı oranlarda karışım olarak kullanılması oldukça yaygın bir kullanım alanı bulmuştur. İlaç üretimindeki maddi ve zamansal kısıtlamalar, tedavi yerine destekleyici bitkisel bileşenlerin kullanımı açısından önemli bir fayda sağlamaktadır. Bununla birlikte, bu bileşenleri oluştururken, bitkilerin kökeninden aktif maddeye ve kullanılabilecek doz aralığına kadar birçok alanda toksik olmayan dozu belirlemek gerekmektedir. Bu çalışmada Remember Regeneration Tedavide (RTM) kullanıldığı bilinen DVD-REG® bitkisel takviyelerinin akut ve subakut toksisite analizleri yapılmıştır. Klinik gözlem bulguları ISO 10993-11:2017 standartlarına göre değerlendirilmiş olup kontrol ve uygulama grupları arasında herhangi bir değişikliğe ratlanılmamıştır. Hematolojik ve biyokimyasal kan parametreleri 0., 1., 7. ve 14. günlerinde değerlendirilmiş kontrol ve uygulama grupları arasında herhangi bir değişiklik tespit edilmemiştir. Histolojik açıdan toksisitenin araştırıldığı karaciğer, kalp, böbrek ve dalak dokularında gruplar arasında patolojik bir bulguya rastlanılmamıştır. Bu çalışma ile Türkiye’de kullanımakta olan gıda takviyesi DVD-REG®’in akut ve subakut etkileri araştırılmış olup elde edilen veriler kronik toksisite testleri için fayda sağlayacaktır.Öğe Effect of My Guard® Food Supplement on Acute and Subacute Toxicity on Rats(Düzce Üniversitesi, 2020) Yaşar, Mustafa; Şenoğul, Oğuzhan; Şirin, Neslihan; Ağan, Aydan Fülden; Sipahi, Nisa; Ağan, KağanObjective: Today, in the field of traditional and complementary medicine, there has been a serious interest in medicinal plants and the treatment methods performed with the use of these plants. The use of natural resources in drug production studies and the promotion of these uses led to the advantage of reaching the drug at a reasonable cost with a rational method. However, these studies should be carried out with objective practices within the framework of scientific ethical rules. Investigating the toxicity dimension in studies related to the subject is also very important for human health to use these products. For this reason, the aim of the study is to determine the toxic symptoms of the My Guard® herbal mixture syrup which is a product used in the remember regeneration therapy method.Material-Method: In the experimental design, there were 4 groups (1 control and 3 application groups). To observe acute and subacute toxicity, clinical observation, the biochemical, hematological and histopathological parameters of the animals were evaluated at the end of the application times. Results: According to the results obtained from the study, there were not found significant differences in biochemical, hematological and histopathological evaluations between the control and the application groups. Conclusion: In our study, the toxic effects of My Guard® product in the dose mentioned in acute and subacute periods were not observed.Öğe Investigation of the Acute Subacute Toxicity of KL21® Supplementary Food Product in Rats(2022) Gök, Ali; Yasar, Mustafa; Saraç, Güliz; Sipahi, Nisa; Ağan, Aydan FüldenObjective: KL21®; It is a food supplement using remember regeneration therapy method (RTM). KL21®. Whereas products that containing combined medicinal plants are expected to be beneficial for health, on the other hand, their toxic properties are able to potentially increase due to the interactions of the active ingredients in the plants. With this study, it was aimed to investigate the toxic effect of KL21®. Material-Method: In the study conducted with control (n: 8), acute (n: 8), subacute (n: 8) and post-subacute (n: 8) groups; the daily dose of the product was tested by gavage in 8-week-old female Wistar rats. Biochemical parameters P, Ca, ALB, TG, TP, TC, Creatinine, Bilirubin, GGT, ALP, AST and ALT were analyzed. In hematological parameters; WBC, RBC, PLT, HCT, HGB were examined. Liver, lung, spleen, kidney and heart tissues were investigated histopathologicaly. Clinical observation was made throughout the entire process. Results: When the acute, subacute and post-subacute groups were compared to control, it was observed that there was no significant difference in biochemical, hematological, histopathological terms. No toxicity-related side effects were found in clinical observations. Conclusion: The potential toxic effect of daily use dose of KL21® containing the combined medicinal plant was investigated in vivo. According to the hemogram, biochemistry and pathology tests, it was determined that it does not show acute and subacute toxicity.Öğe MELİTTİN VE KANSER TEDAVİSİ: NANOTEKNOLOJİK BAKIŞ AÇISI(2020) Ağan, Aydan Fülden; Kekeçoğlu, MeralKanser, insan sağlığını tehdit eden en önemli sağlık sorunlarından birisi olmaya devam etmektedir. Günümüzde kanser hastalığını tedavilerinde cerrahi yöntemler, kemoterapi, radyoterapi, immunoterapi, kanser aşıları gibi yöntemler kullanılmaktadır. Ancak başta kemoterapi olmak üzere bu tedavi yöntemlerinin bazı yan etkilere neden olması, doğal ürünlere olan ilgiyi arttırmıştır. Arı zehrinin peptit bileşenlerinden biri olan melittin, kanser tedavisinde kullanılan en önemli etken maddelerinin başında gelir. Melittinin anti-kanser etkinliği daha önce yapılan araştırmalarla çeşitli kanser türlerinin tedavisinde pre-klinik olarak test edilmiştir. Ancak bu pre-klinik sonuçların kliniğe uygulanabilmesi için, başta melittinin neden olduğu hemolitik aktivite, spesifik olmayan toksisite ve bozunma gibi sorunların üstesinden gelinmelidir. Bu amaçla melittinin çeşitli nanopartiküller ile bir araya getirilerek, melittinin kanserli hücreyi hedefleyebileceği platformlar sunulmuştur. Bu derlemede, henüz çok güncel ve halen geliştirilmekte olan arı zehri bileşenlerinin çeşitli kanser türlerinin tedavisine yönelik in-vitro ve in-vivo uygulamalarına dair mevcut literatür, nanoteknolojik uygulamalar açısından bir araya getirilmiştir.Öğe Systemic Investigation of Acute Toxicity of Some Food Supplements on The Market in Turkey(Düzce Üniversitesi, 2020) Yaşar, Mustafa; Şenoğul, Oğuzhan; Gök, Ali; Ağan, Kağan; Ağan, Aydan Fülden; Beyazçiçek, Ersin; Kantarcıoğlu Coşkun, SinemObjective: Along with changing living and working conditions in recent years, changes have occurred in eating habits. People are turning to supplements for many purposes such as supplementing normal nutrition, feeling more energetic, enhancing the immune system by taking vitamins and minerals that they think they do not get enough, and meeting their body's needs when they cannot eat a balanced and healthy diet. Supplements are prepared in various forms using nutrients such as vitamins, minerals, proteins, amino acids, plants, enzymes, fiber and fatty acids. These supplement foods are not intended to treat or prevent any disease. In general, supplement foods; it is not a substitute for natural nutrition, but it is intended to complement its deficiencies. They cannot be recommended and sold instead of medicines. Because of many of these products have natural content, they can be placed on the market without toxicity tests. In the current study, it is planned to observe the effects of acute systemic toxicity of supplements that are used in remember regeneration therapy method (RTM) and sold in the markets with the brands’ name of IST-ARD®, ARD-REM®, DVD-ARD®, KT-REM®, ARDZ-REM®, IST-GLIO®, IST-REM®, ROMX®, and REGULIN®. Material-Method: In the experimental design, there were 10 groups (1 control and 9 application groups) male mice. To observe acute toxicity, clinical observation was performed for 72 hours and the biochemical and histopathological parameters of the animals were evaluated at the end of the application. Results: According to the results obtained from the study, there were not found significant differences in biochemical and histopathological evaluations between the control and the application groups. Conclusion: The acute toxicity effect of the food supplements was not determined. May be further studies with bigger numbers of samples investigating the nephrotoxic effects of these products lighten this matter.
