Bodur, Akınİnce, İmranKahraman, CemilAbidin, İsmailAbidin, Selcen AydınAlver, Ahmet2020-05-012020-05-0120190003-98611096-0384https://doi.org/10.1016/j.abb.2019.02.004https://hdl.handle.net/20.500.12684/5674Bodur, Akin/0000-0001-7413-2717; kahraman, cemil/0000-0002-4494-6063WOS: 000464892400007PubMed: 30797748The purpose of this study was to investigate the effects of a high fat and a high sucrosediet in wild type and BDNF (+/-) mice on oxidative stress in epididymal and subcutaneousadipose tissues by measuring different markers of oxidative stress and antioxidant enzymes. Wild type (WT) and BDNF (+/-) male mice were divided into six groups receiving fed control diet (CD), high sucrose diet (HSD), or high fat diet (HFD) for four months. Levels of 3-nitrotyrosine (3-NT) increased in the HFD-fed BDNF (+/-) mice, while 4-hydroxynonenal (4-HNE) levels increased in the CD and HFD-fed BDNF (+/-) groups. Malondialdehyde (MDA) levels decreased in subcutaneous tissue compared to epididymal adipose tissue, independently of diet type. Superoxide dismutase (SOD) activity was reduced by HFD (p < 0.05), butglutathione peroxidase (GSH-Px) activity was increased by HSD in epididymal adipose tissuein BDNF (+/-) mice (p < 0.05). GSH-Px activities was increased by CD and HFD in subcutaneous adipose tissue of BDNF (+/-) (p < 0.05). SOD2 and GSH-Px3 expressions were only decreased by HSD in epididymal and subcutaneous adipose tissues of BDNF (+/-) mice (p < 0.05). In conclusion, reduced BDNF may increase OS in epididymal adipose tissue, but not in subcutaneous adipose tissue following HSD and HFD.en10.1016/j.abb.2019.02.004info:eu-repo/semantics/closedAccessBDNFEpididymal adipose tissueSubcutaneous adipose tissueOxidative stressHigh sucrose dietHigh fat dietArticle6654656WOS:000464892400007Q1Q2