Arslan, Gülgün ÇakmakSevercan, MeteZorlu, FarukSevercan, Feride2020-04-302020-04-3020160955-30021362-3095https://doi.org/10.1080/09553002.2016.1230237https://hdl.handle.net/20.500.12684/4661Zorlu, Faruk/0000-0002-9835-4768; Cakmak-Arslan, Gulgun/0000-0003-4326-1780WOS: 000388720200010PubMed: 27585945Purpose: To investigate the effects of whole body ionizing radiation at a sublethal dose on rat brain homogenate membranes and the protective effects of amifostine on these systems at molecular level. Materials and methods: Sprague-Dawley rats, in the absence and presence of amifostine, were whole-body irradiated at a single dose of 8Gy and decapitated after 24 h. The brain homogenate membranes of these rats were analyzed using Fourier Transform Infrared (FTIR) spectroscopy. Results: Ionizing radiation caused a significant increase in the lipid to protein ratio and significant decreases in the ratios of olefinic = CH/lipid, CH2/lipid, carbonyl ester/lipid and CH3/lipid suggesting, respectively, a more excessive decrease in the protein content and the degradation of lipids as a result of lipid peroxidation. In addition, radiation changed the secondary structure of proteins and the status of packing of membrane lipid head groups. Furthermore, it caused a decrease in lipid order and an increase in membrane fluidity. The administration of amifostine before ionizing radiation inhibited all the radiation-induced alterations in brain homogenate membranes. Conclusions: The results revealed that whole body ionizing radiation at a sublethal dose causes significant alterations in the structure, composition and dynamics of brain homogenate membranes and amifostine has a protective effect on these membranes.en10.1080/09553002.2016.1230237info:eu-repo/semantics/closedAccessAmifostinebrain homogenate membraneFTIR spectroscopyionizing radiationlipid peroxidationprotein secondary structureArticle9212837848WOS:000388720200010Q2Q1