Karabörklü, SalihAltın, NedimKeskin, Yeşim2020-04-302020-04-3020190031-7454https://hdl.handle.net/20.500.12684/3767Karaborklu, Salih/0000-0003-4737-853XWOS: 000467889200009Native entomopathogenic fungi were isolated from Duzce province of Turkey and their virulence was tested against Tenebrio molitor larvae using direct spraying and grain spraying methods. Forty-five isolates were obtained. Beauveria bassiana was obtained from 24.07% of the soil samples and Metarhizium anisopliae from 11.11% of the soil samples. The level of insecticidal activity of the isolates fluctuated between 20% and 100%, when directly sprayed on the larvae. In the direct spray application, the most effective isolates were B. bassiana YK11, YK16, M. anisopliae YK43, YK44 and YK45. These isolates caused 100% mortality on the larvae of T. molitor at 168 h. In 12 isolates, there was 100% mortality at 240 h under laboratory condition. Lethal time (LT50) of B. bassiana and M. anisopliae isolates varied from 10.48 to 127.40 h. Virulence of the fungal isolates in 3% (w/v) aqueous suspension was lower than that in the 10% aqueous (w/v) suspension in the grain spray application. Virulence of fungal isolates was significant in 10% aqueous suspension and 93.33% and 100% mortality were observed in B. bassiana YK23 and YK26, respectively, at 240 h. B. bassiana and M. anisopliae isolates showed promising effectiveness for the control of insect pests.eninfo:eu-repo/semantics/closedAccessBeauveriaMetarhiziumTenebrio molitorvirulencewheat grainArticle10218289WOS:000467889200009Q4