Botsali, AysenurErbil, HakanEsme, PelinGamsizkan, MehmetAksoy, Ali OkanCaliskan, Ercan2024-08-232024-08-2320231076-05121524-4725https://doi.org/10.1097/DSS.0000000000003874https://hdl.handle.net/20.500.12684/14165INTRODUCTION Biostimulation properties of diluted and hyperdiluted calcium hydroxylapatite (CaHA) injections have become increasingly popular. However, the existing data are insufficient to certify a particular dose-response pattern.OBJECTIVE To assess and compare the dermal stimulation potentials of different concentrations of CaHA injections.MATERIALS AND METHODS Two independent experiments (Experiment-1: constant injection volume vs Experiment-2: constant CaHA amount) included 4 study groups each, and these experimental groups were placed consecutively on the abdominal skin of a juvenile Yorkshire pig. Histopathological and immunohistochemical stainings were performed on punch biopsy materials collected 4 months after the injection day.RESULTS The fibroblast count significantly decreased upon dilution from 1:3 to 1:19 in experiment 1 (p = .000) but still higher than the control group. In experiment 1, the collagen density of the concentrated form was more elevated than the 1:19 dilution and the negative control groups (p = .034 and .000, respectively) but similar to the 1:3 dilution (p = .123). No significant difference was observed between the groups regarding collagen density with a standard amount of CaHA (0.2 mL, 30%) (p > .05).CONCLUSION Despite the efficacy being more pronounced till 1:3 dilution, hyperdiluted CaHA at any dilution ratio up to 1:19 can provide a higher fibroblast count than the negative control group.en10.1097/DSS.0000000000003874info:eu-repo/semantics/closedAccessNeocollagenesisArticle499871876373991372-s2.0-85169172580WOS:001058050200010Q2Q1