Ozyigit, Ibrahim IlkerFiliz, ErtugrulSaracoglu, Ibrahim AdnanKaradeniz, Sedat2021-12-012021-12-0120201310-28181314-3530https://doi.org/10.1080/13102818.2020.1773311https://hdl.handle.net/20.500.12684/10818Boron (B) is an essential plant micronutrient but studies regarding its transport are still limited to a few plants. This work identified two major B transport sequences in plants, NIP5;1 boric acid channel protein and BOR1 transporter. 80 BOR1 and 34 NIP5;1 homologs were identified in 18 different plant genomes. BOR1 homologs had a HCO3-transporter domain, 649-737 amino-acid residues with mainly basic nature, putative 8-11 transmembrane domains (TMDs) and 11-13 exons. NIP5;1 homologs had a MIP family domain, 294-311 amino-acid residues with basic nature, 5-6 putative TMDs and 3-5 exons. Tyrosine-based motif, acidic di-leucine motif and lysine residue, reported for polarity, vacuolar sorting and B-dependent degradation, were identified in BOR1 homologs. Two NPA motifs and an ar/R selectivity filter with AIGR residues, reportedly essential in B transport, were also found in NIP5;1 homologs. Two NPA motifs in AtNIP5;1 and OsNIP3;1 homologs were NPS and NPV, whereas in sequences homologous to AtNIP6;1 were NPA/V. Besides, ar/R selectivity filters were identified with A(N/S/T)IGR residues in NIP5;1 and NIP3;1 homologs. The BOR1 and NIP5;1 model structures were mainly conserved. Under different perturbations, Arabidopsis thaliana NIP5;1 and NIP6;1 genes demonstrated similar expression patterns although they act in different tissues, suggesting a common regulatory mechanism, whereas BOR1 showed a different expression pattern. BOR1 was substantially expressed in primary root, radicle and flower; NIP5;1 in primary root and roots, and NIP6;1 in petiole. NIP5;1, 6;1 and BOR1 expression in other plant organs implied their involvement in different pathways in addition to B uptake and its mobilization.en10.1080/13102818.2020.1773311info:eu-repo/semantics/openAccessBoric acidorthologueparaloghomologmotifselectivity filterEfflux TransporterInflorescence DevelopmentReproductive DevelopmentRhamnogalacturonan IiWide IdentificationCross-LinkingCell-WallsRolesExpressionDeficiencyArticle3414554682-s2.0-85087093473WOS:000538924900001Q3Q4