Angelini, ClaudioVizzini, AlfredoJusto, AlfredoBizzi, AlbertoDavoli, PaoloKaya, Ertugrul2021-12-012021-12-0120201664-302Xhttps://doi.org/10.3389/fmicb.2020.01833https://hdl.handle.net/20.500.12684/10759A recent collection ofLepiota spiculatafrom the Dominican Republic is presented here. Macro- and micromorphological features ofL. spiculataare described in detail, and its evolutionary (phylogenetic) position withinLepiotasect.Ovisporae, in the subincarnata/brunneoincarnata clade, is assessed on the basis of a combined nrLSU + nrITS +rpb2+tef1analysis. Additionally, high levels of deadly amatoxins were detected and quantified inL. spiculatafor the first time by HPLC analysis; in particular, alpha-amanitin was found at concentrations up to approximately 4 mg/g dry weight, which renderL. spiculataa potentially lethal mushroom, if ingested.en10.3389/fmicb.2020.01833info:eu-repo/semantics/openAccessAgaricomycetesAgaricaceaeLepiotamolecular and chemical analysestaxonomytoxinspoisoning riskFulminant Hepatic-FailureCyclopeptide ToxinsBrunneoincarnataAmatoxinsMushroomIdentificationArticle112-s2.0-85089911346WOS:000565294500001Q1Q1