Kaya, Mustafa OguzhanDemirci, TunaÖzdemir, OğuzhanÇalışır, ÜmitSönmez, FatihArslan, Mustafa2023-07-262023-07-2620231054-25231554-8120https://doi.org/10.1007/s00044-023-03029-7https://hdl.handle.net/20.500.12684/12507The novel sulfonamide substitute 1,4-dihydropyridine derivatives were synthesized by the method of Hantzsch reaction. They have been characterized by FT-IR spectroscopy, H-1-NMR, C-13-NMR, and elemental analysis. PON1 which is an antioxidant enzyme has important functions in cardiovascular systems. The enzyme has been purified using a two-step method such as ammonium sulfate precipitation and sepharose-4B-l-tyrosine-9-aminophenanthrene hydrophobic interaction chromatography. The results demonstrated that all the synthesized compounds inhibited PON1 enzyme. The best inhibition effect was observed in compound (1) for PON1 enzyme (IC50: 8.04 mu M, K-i: 5.43 mu M). The free radical scavenging for PON1 was discovered as 20.16 mg/mL, while drug score value was reported as 0.13 for compound (1). Furthermore, the lowest binding energy (-1.31 kcal/mol) determined by molecular docking for PON1 enzyme and the lowest LUMO-HOMO gap ( increment E = 3.12 eV) were calculated for compound (1).en10.1007/s00044-023-03029-7info:eu-repo/semantics/closedAccessPon1; Inhibition; Drug Score; Molecular Docking; 1; 4-DihydropyridinePharmacological-Activities; Drug Discovery; PurificationArticle2-s2.0-85149012757WOS:000942171600003Q2Q3