Uğraş, Serpil2020-04-302020-04-3020171010-6960https://doi.org/10.16970/ted.74860https://hdl.handle.net/20.500.12684/3527WOS: 000418508400001Honeybees are considered as a key species in nature for their vital role in the maintenance of almost all life on earth. However, the massive death of honeybee stocks worldwide, largely due to colony collapse disorder, is causing international concern. In order to avoid these losses, new approaches must be sought. In previous studies, the probiotic properties of the bacteria found in the bodies of honeybees are thought to have an active role in providing resistance against pathogens. Consequently, in this study, it is aimed to isolate probiotic lactic acid bacteria from honey stomachs of the healthy honeybee, to examine the effect of these bacteria against pathogenic bacteria and to use these bacteria to boost the immune system of bees. For this purpose, between 2015 and 2016, probiotic bacteria were screened from honey bees that provided by DAGEM (Duzce University, Beekeeping Research, Development and Application Center, Yigilca, DUZCE). The inhibitory activity of the obtained bacteria against the bee pathogen Melissococcus plutonius (Truper and de 'Clari, 1998) (Enterococcaceae) was determined by in vitro agar well diffusion. The bacterium with the desired characteristics were identified by biochemical, physiological and 16s rDNA analysis as Lactobacillus kunkeei (Edwards, 1998) (Lactobacillaceae) and its probiotic nature was investigated. With the evaluation of these findings, future preparations of the isolate are expected to support the bee immune system and, as a result, to produce resistant honeybees without resorting to treatment with antibiotics.en10.16970/ted.74860info:eu-repo/semantics/closedAccessBacteriumhoneybeeisolationLactobacillus kunkeeiprobioticsArticle413253261WOS:000418508400001Q3Q4