Erdogan, ZiyaOzturk, Cihadiye ElifCangur, SengulCaliskan, EmelOksuz, SukruKaramurat, Zeynep Dilara2021-12-012021-12-0120211309-3878https://doi.org/10.18521/ktd.834769https://hdl.handle.net/20.500.12684/10271Objective: An irreversible process begins when a systemic infection causes sepsis. Therefore, rapid identification of the agent bacteria in sepsis and its antibiotic resistance is crucially important. In this study, it was aimed to investigate the efficiency of rapid genotype test in detecting sepsis agent Gram positive bacteria and important antibiotic resistance. Methods: 2132 blood culture samples sent to the laboratory were examined with an automatic blood culture system (BACTEC, BD, USA) between 2018-2019. Blood culture bottles sent to the laboratory were Growing bacteria was identificated by VITEK (bioMerieux, France) automated bacteria identification / antibiotic susceptibility system. In addition, bacterial species and mecA, vanA, vanB, vanC1, vanC2 / C3 genes in blood cultures with Gram positive bacterial growth were also determined by the Genotype (R) BC Gram-positive (Hain Lifesience, Germany) test. Results: 72 patients with gram-positive bacteria growth in two or more blood culture bottles were included in the study. In 44 of the samples (61%) the same bacterial species were detected with conventional method (bacteria culture) and BC Gram positive test. In 28 of the samples (39%) differences were detected between results of methods regarding bacterial species name or vancomycin/methicillin resistance rate. Although single agent was isolated with culture method in all of the samples, multiple agents were detected in eight samples with rapid genotype test. Also, it was found that in mecA positive samples, ciprofloxacin resistance was higher than mecA negative ones. Conclusions: In the study, it was observed that BC Gram positive test could correctly identify sepsis agent bacteria and their resistance genes within 4-5 hours.en10.18521/ktd.834769info:eu-repo/semantics/openAccessDrug ResistancePCRRapid testSepsisStaphylococciResistant Staphylococcus-AureusBlood Culture BottlesTime Pcr AssayVan GenesMecaInfectionsPathogensArticle133511518WOS:000709040200004N/A