Yaşar, MustafaŞenoğul, OğuzhanKaradeniz, BesteGök, AliAğyar Yoldaş, PınarAğan, Kağan2024-12-282024-12-2820202717-7491https://dergipark.org.tr/tr/download/article-file/1121327https://dergipark.org.tr/tr/pub/ijtcmr/issue/56526/743285https://hdl.handle.net/20.500.12684/17184Objective: Recently, there is a growing interest in medicinal and aromatic plants also the treatment methods by using these plants in traditional and complementary medicine. On the other hand, possible toxicities of medicinal and aromatic plants has lately begun to be researched and it is extremely important to cognize these possibilities for human consumption. Therefore, a research has been conducted to understand the acute/subacute toxicity characteristics of a plant based herbal mixture, ISY-CP® used in remember regeneration therapy method (RTM) in rats. The product contains a mixture of nettle leaves (Urtica dioica), yarrow (Achillea millefolium), thyme (Thymus vulgaris) and horsetail (Equisetum arvense) plants. The aim of this research was to examine the effects of ISY-CP® herbal mixture in term of toxicity. Material-Method: In the experimental design, 32 female rats were divided into 4 groups (control, acute, subacute and post-subacute). In order to observe acute and subacute toxicity, clinical observations were performed and the biochemical, hematological and histopathological parameters of the animals were evaluated at the end of the application. Results: According to the results obtained from the study, there were not found significant differences in biochemical, hematological and histopathological evaluations between the control and the application groups. Only phorphorus data is statistically different between subacute toxicity group and control group.Conclusion: In our study, the acute and subacute toxic effects of ISY-CP® herbal mixture’s doses used in this study were not observed.eninfo:eu-repo/semantics/openAccessTraditional and complementary medicineacute toxicitysubacute toxicityUrtica dioicaAchillea millefoliumThymus vulgarisEquisetum arvenseTraditional, Complementary and Integrative MedicineArticle125460DergiPark: 743285